oriTDB

The information of the oriT region

oriTDB accession number	100171
Name	oriT_pWES-1
Sequence Completeness	incomplete
oriT length	12 nt
oriT sequence	AGCAAGGTGTAA
IR (inverted repeat)	_
Location of nic site	_
Conserved sequence flanking the nic site	_
Note	similar to Bacteroides fragilis cLV25 chromosomal transfer factor nic site; putative nic site of oriT
	D:

Visualization of oriT structure

Reference

[1] Doublet B et al (2009) Novel plasmid-encoded ceftazidime-hydrolyzing CTX-M-53 extended-spectrum beta-lactamase from Salmonella enterica serotypes Westhampton and Senftenberg. Antimicrob Agents Chemother. 53(5):1944-51. [PMID:19273683]

I. Information of Relaxase

ID	164
Name	Orf2_pWES-1
GenBank accession number	ACJ65155
Family	MOB_P
Length	813 aa
UniProt ID	B7NZZ4
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.50E-20, Aligned region: 8..255
Note	Relaxase/mobilization nuclease topoisomerase/primase fusion protein
Protein sequence [Download]	MKAKVSRGGGFRGALNYVFDVGKEATHTKNAERVGGNMAGNDPRELSREFSAVRQLRPDI GKPVWHCSLSLPPGERLSAEKWEAVAADFMQRMGFDQTNTPWVAVRHQDTDKDHIHIVAS RVGLDGKVWLGQWEARRAIEATQELEHTHGLTLTPGLGDARAERRKLTDKEINMAVRTGD EPPRQRLQRLLDEAVKDKPTALELAERLQAAGVGVRANLASTGRMNGFSFEVAGVPFKGS DLGKGYTWAGLQKAGVTYDEARDRAGLERFRPTVADRGERQDVAAVREPDARGLEAPTGR SLDRDGADLGTAGPTPAGRDAGSGSLRQGDGHSAQDAGRADAADERERGAGLRAEGREAG RDHLRPVAQPVRAENEPQQHGADRAAGGDLAGQAGERTAGHDESRRPTDRGSERDAPAPL AAGAGADSGRGRGRDAGSDWASRFKQASAAKRRAADGRLGQRDLEQGHAHGARVAETDRQ SARELDPTAYLEASGYTVKREGRHLSVRAGGDEAYRVTRQQDGRWLWCDRYGNDGGDNID LVREIEPGTGYAEAVYRLSGAPTVRQQPRPSEPKRQPPQLPAQGLAAREHGRDYLKGRGI SQDTIEHAEKAGMVRYADGGVLFVGYDRAGTAQNATRRAIAPADPVQKRDLRGSDKSYPP ILPGDPAKVWIVEGGPDALALHDIAKRSGQQPPTVIVSGGANVRSFLERADVQAILKRAE RVTVAGENEKNPEAQAKADAGHQKQAQRVAKITGREVRQWTPKPEHGKDLADMNARQVAE IERKRQAEIEAERARNRELSRKSRRYDGPSFGR

Reference

[1] Doublet B et al (2009) Novel plasmid-encoded ceftazidime-hydrolyzing CTX-M-53 extended-spectrum beta-lactamase from Salmonella enterica serotypes Westhampton and Senftenberg. Antimicrob Agents Chemother. 53(5):1944-51. [PMID:19273683]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	236	MobC_pWES-1	ACJ65154	128 aa	auxiliary mobilization protein C

N/A

Information of plasmid

ID	164
Plasmid name	pWES-1
GenBank accession number	DQ268764.2
Incompatibility group	IncQ-like
Genome size	10908 bp
Coordinate of oriT [Strand]	2659..2670 [+]
Drug resistance	CTX-M-53 beta-lactamase encoded by bla_CTX-M-53 gene; resistance to
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Salmonella enterica serovar Westhampton [356621]

Reference

[1] Doublet B et al (2009) Novel plasmid-encoded ceftazidime-hydrolyzing CTX-M-53 extended-spectrum beta-lactamase from Salmonella enterica serotypes Westhampton and Senftenberg. Antimicrob Agents Chemother. 53(5):1944-51. [PMID:19273683]