oriTDB
The information of the oriT region
oriTDB accession number 100168
Name oriT2_pNZ4000  insolico
Sequence Completeness intact
oriT length 416 nt
oriT sequence CATGGTTTTAGGAAAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTCTTC TTTTTGTATGACAAAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGTAGGTG GCGTTTGCGTAAAGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTC CCCCTTTTCAAGCAACATTGTAATACAAGAACGAAGTGATTTGTATTACAATGTGATAGC TTGCAGTATTTATGGTTTTATATGGTCTATTTTGTTGTGAGGATTGTAACCGAATAGGGC GCAATACTTATTACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGAGGCATTT TATCTTTGAGTAAGTTATTGATGGATCAGAAAAATGTATCACAAATTGAAACAAAG
IR (inverted repeat)IR1: [91-100] [105-114] nt  (CCCATTTTAT..ATAAAATGGG)
IR2: [160-172] [176-188] nt  (AAAAGGGGAAAGT..ACTTCCCCCTTTT)
IR3: [196-208] [221-233] nt  (CATTGTAATACAA..TTGTATTACAATG)
Location of nic site [243-244] nt
Conserved sequence flanking the
  nic site 		CTTG|CA
Note _
L:91-100;R:105-114;L:160-172;R:176-188;L:196-208;R:221-233;D:243-244
Visualization of oriT structure
Reference
[1] van Kranenburg R et al (1998) Characterization of multiple regions involved in replication and mobilization of plasmid pNZ4000 coding for exopolysaccharide production in Lactococcus lactis. J Bacteriol. 180(20):5285-90. [PMID:9765557]
I. Information of Relaxase
ID 161
Name MobA_pNZ4000 insolico
GenBank accession number NP_053037
Family MOBP
Length 410 aa
UniProt ID O54677
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 1.20E-74, Aligned region: 9..280
Note relaxase
Protein sequence [Download] MATIAKISNGASAASALNYALGQDRPMHEKTEQWLHDHQLERPVELTNCRAVAVGGTNGI
DPFIAKEQFDVVRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLY
PNHQSAVYTHLDGKNHVLHNHIIVNKVNLETGKKLREQKGESVQRAREMNDRLASRENWH
ILEPPKERQTETEKELIAKNEYSYMNDLRERINKSLQDVSVSSYETFKERLSDNGVILSE
RGQTFSYAFLDANNKQRRARETRLGSDFGKETILHELENRARQNEFSAVEQREPAITPLE
RDTQQRESEIVSLEQAIEPRKSEARKRESKINRFIDTIKQFAGRVPELTQRVTRKLKQTK
EKILDDFERRFSKDMKNYEQEQQKSLEKQANRDVQSEKKPTKDHDRGMSR
Reference
[1] van Kranenburg R et al (1998) Characterization of multiple regions involved in replication and mobilization of plasmid pNZ4000 coding for exopolysaccharide production in Lactococcus lactis. J Bacteriol. 180(20):5285-90. [PMID:9765557]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 232 MobB_pNZ4000 insolico NP_053038 207 aa putative mobilization protein
2 233 MobC_pNZ4000 insolico NP_053039 200 aa putative mobilization protein
 N/A
Information of plasmid
ID 161
Plasmid name pNZ4000
GenBank accession number NC_002137.1
Genome size 42810 bp
Coordinate of oriT  [Strand] 28620..29037 [+]; 11149..11564 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Lactococcus lactis subsp. cremoris NIZO B40 [1359]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU