 oriTDB accession number | 100167 |
| Name | oriT1_pNZ4000  |
Sequence Completeness  | intact |
| oriT length | 418 nt |
| oriT sequence | CCATGGTTTTAGAAAAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTTCT TCTTTTTGTATGACAAAAAGAAAGATTTTTTGCCCATTTTATTTTTATAAAATGGGTAGG TGGCGTTTGCGTAAAGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACT TCCCCCTTTTCAAGCCACATTGTAATACAAGAACGAAGTGATTTGTATTACAATGTGATA GCTTGCAGTATTTATGGTTTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGG GCGCAATGCTTATTACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCAT TTTATCTTTGAGGAGGCTATTTATGGATCAGAAAAATGTATCACAAAATCAAACAAAG |
| IR (inverted repeat) | IR1: [93-102] [107-116] nt (CCCATTTTAT..ATAAAATGGG) IR2: [162-174] [178-190] nt (AAAAGGGGAAAGT..ACTTCCCCCTTTT) IR3: [196-210] [223-237] nt (CACATTGTAATACAA..TTGTATTACAATGTG) |
| Location of nic site | [245-246] nt |
| Conserved sequence flanking the nic site | CTTG|CA |
| Note | _ |
Visualization of oriT structure Reference| ID | 161 |
| Name | MobA_pNZ4000 |
| GenBank accession number | NP_053037 |
| Family | MOBP |
| Length | 410 aa |
| UniProt ID | O54677 |
| PDB ID | _ |
| Pfam | Relaxase [PF03432.13], Evalue: 1.20E-74, Aligned region: 9..280 |
| Note | relaxase |
| Protein sequence [Download] | MATIAKISNGASAASALNYALGQDRPMHEKTEQWLHDHQLERPVELTNCRAVAVGGTNGI DPFIAKEQFDVVRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLY PNHQSAVYTHLDGKNHVLHNHIIVNKVNLETGKKLREQKGESVQRAREMNDRLASRENWH ILEPPKERQTETEKELIAKNEYSYMNDLRERINKSLQDVSVSSYETFKERLSDNGVILSE RGQTFSYAFLDANNKQRRARETRLGSDFGKETILHELENRARQNEFSAVEQREPAITPLE RDTQQRESEIVSLEQAIEPRKSEARKRESKINRFIDTIKQFAGRVPELTQRVTRKLKQTK EKILDDFERRFSKDMKNYEQEQQKSLEKQANRDVQSEKKPTKDHDRGMSR |
Reference| [1] van Kranenburg R et al (1998) Characterization of multiple regions involved in replication and mobilization of plasmid pNZ4000 coding for exopolysaccharide production in Lactococcus lactis. J Bacteriol. 180(20):5285-90. [PMID:9765557] |
| # | ID | Name | GenBank | Length | Note |
| 1 | 232 | MobB_pNZ4000 | NP_053038 | 207 aa | putative mobilization protein |
| 2 | 233 | MobC_pNZ4000 | NP_053039 | 200 aa | putative mobilization protein |
| ID | 161 |
| Plasmid name | pNZ4000 |
| GenBank accession number | NC_002137.1 |
| Genome size | 42810 bp |
| Coordinate of oriT [Strand] | 28620..29037 [+]; 11149..11564 [-] |
| Drug resistance | _ |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Lactococcus lactis subsp. cremoris NIZO B40 [1359] |
Reference