 oriTDB accession number | 100151 |
| Name | oriT_pColK-K235  |
Sequence Completeness  | incomplete |
| oriT length | 33 nt |
| oriT sequence | GTAGCGATAGCGGAGTGTATACTGGCTTAATCA |
| IR (inverted repeat) | _ |
| Location of nic site | [25-26] nt |
| Conserved sequence flanking the nic site | CTGG|CTTA |
| Note | ColE1 superfamily |
Visualization of oriT structure Reference| [1] Rijavec M et al (2007) Prevalence of ColE1-like plasmids and colicin K production among uropathogenic Escherichia coli strains and quantification of inhibitory activity of colicin K. Appl Environ Microbiol. 73(3):1029-32. [PMID:17122402] |
| ID | 145 |
| Name | MbkA_pColK-K235 |
| GenBank accession number | YP_214176 |
| Family | MOBP |
| Length | 532 aa |
| UniProt ID | Q58P29 |
| PDB ID | _ |
| Pfam | Relaxase [PF03432.13], Evalue: 1.40E-18, Aligned region: 49..247 |
| Note | relaxase |
| Protein sequence [Download] | MIVKFHARGKGGGSGPVDYLLGRERNREGARVLRGAPEEVRELIDATPFAKKYTSGVLSF AEQTLPPGERERVMESFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELASGKRL QPYYDRADRPRINAWQTLVNHHYGLHDPNAPENRRTLVTPNNLPKAKQEAAEAITRGLEA LYHAGALKTRQDVTEALTAAGFEVVRTTRSSISIADPEGGRNLRLKGALYEQSFTNGDRL REETERACRLYREHAEQRVQEARSVCRQGTELKRAENQRRYQRTHSSGERTTGDAEQCRE RTVQRHRERTGPAAGRAALEAGQRADTVPVADGALRHGRDFSGVMGADSVAGKPDCGKHP GYQGPGRDAGETPGKNMGCDVSRGQEREISGAAVRGEGGKQLDGGEEERSKTGQGVSEYD RAGNALTERLRAIAAGLYAAAERLGEPLHGFAAYVRADTTGERATEGTVRRLESAGAEIS RAGGPLESVIYHEQQIRDEQERQARLERERARSWHEEQEREARVWRGPSIGM |
Reference| [1] Rijavec M et al (2007) Prevalence of ColE1-like plasmids and colicin K production among uropathogenic Escherichia coli strains and quantification of inhibitory activity of colicin K. Appl Environ Microbiol. 73(3):1029-32. [PMID:17122402] |
| # | ID | Name | GenBank | Length | Note |
| 1 | 210 | MbkB_pColK-K235 | YP_214177 | 186 aa | MbeB-like, N-term conserved region; pfam04837 |
| 2 | 211 | MbkC_pColK-K235 | YP_214175 | 67 aa | mobilization protein |
| ID | 145 |
| Plasmid name | pColK-K235 |
| GenBank accession number | NC_006881.1 |
| Genome size | 8318 bp |
| Coordinate of oriT [Strand] | 5471..5503 [-] |
| Drug resistance | _ |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Escherichia coli [562] |
Reference