 oriTDB accession number | 100145 |
| Name | oriT_pCT14  |
Sequence Completeness  | incomplete |
| oriT length | 30 nt |
| oriT sequence | ATTGCGTATGGTGTATAGCTCGTTAAGGGA |
| IR (inverted repeat) | _ |
| Location of nic site | [14-15] nt |
| Conserved sequence flanking the nic site | GGTGT|ATAGC |
| Note | _ |
Visualization of oriT structure Reference| [1] Bramucci M et al (2006) Genetic organization of a plasmid from an industrial wastewater bioreactor. Appl Microbiol Biotechnol. 71(1):67-74. [PMID:16244860] |
| ID | 139 |
| Name | TraI_pCT14 |
| GenBank accession number | YP_001966297 |
| Family | MOBF |
| Length | 984 aa |
| UniProt ID | Q2QCQ9 |
| PDB ID | _ |
| Pfam | TrwC [PF08751.10], Evalue: 2.00E-84, Aligned region: 12..293 AAA_30 [PF13604.5], Evalue: 1.50E-40, Aligned region: 494..681 Viral_helicase1 [PF01443.17], Evalue: 1.90E-05, Aligned region: 514..704 Viral_helicase1 [PF01443.17], Evalue: 6.70E-06, Aligned region: 792..916 UvrD_C_2 [PF13538.5], Evalue: 2.10E-07, Aligned region: 868..914 |
| Note | relaxase |
| Protein sequence [Download] | MLDITTISRQSLGKVVSYYADGADDYYAKDGGAMQWQGAGAEALGLSGEVEQARFRELLD GRISDSTKLMRTVKEADGKVVSKERLGYDLTFSAPKGVSLQALVHGDASIIEAHDKAVAA AIREAERLSQARITVNKKTGTENTNNLVVAKFRHETSRALDPDLHTHAFVLNMTQRSDGE WRALKNDGVFNSSMFLGNVYKAELARELEKAGFQLRYERNGTFDLAHFSDEQIREFSSRS QQIEAALAAKGLDRSTASYAEKNQAALATRDKKQGGIDREELRQVWLERSRALGIDYHSR EWAGVGADAQGGRERNSAATPQIEKPLEYRADQVIEFAIKSLTERQAVIGQKELMDTALR HGYGALTIDDVRAGIERRAASGHLIREEPLYSSQNPADGKKGKAAEKAREEAPQLSRKEW VATLVRAGKSRSEAARLVDEGIRTGRLRQGENRFTTHIAQKREREVLQIERMGRGTVEPR ISKEAAEAFLADRGLKAEQQASVMRIARTQNQFIGVQGFAGVGKSYMTVAAKDLLEANGY RVTSLAPYGSQVKALQAEGLEARTLQSFLKARDKKIDSNTVVFIDEAGVIPARQMHEAMK TIEAAGARVVFLGDVAQTKAIEAGKPFEQLMKAGMETSRLTDIQRQKDPQLLEAVKLAAE GKAKQSLPLVNEIREIKEDGARYQAIVDAYAKMTKAERDQALIITGTNASRIQINEGVRE ALGLKGQGAEYPLLNRLDTTQAERRHSKYYGKGSIVVPEVDYKNGLQRGVQYVVLDTGPG NKLTVRGPEGETLQFTPARCTKLSVYSVERTELVAGDQVKITRNDAEKDLANGDRFVVKE IHSDRVVLEGDKGRQVELDTASAMFVGLAYASTVHSAQGLTCDKVLINLETQSRTTAKDV YYVAISRARHAAEIFTDNRQKLGDAISRLNAKAGALDIKQLQRHALERKGHDQAGKQKDA VQKQQQGQQLPAKQPKEKGRAFGL |
Reference| [1] Bramucci M et al (2006) Genetic organization of a plasmid from an industrial wastewater bioreactor. Appl Microbiol Biotechnol. 71(1):67-74. [PMID:16244860] |
| # | ID | Name | GenBank | Length | Note |
| 1 | 204 | _ |
| ID | 139 |
| Name | TraJ_pCT14 |
| SecReT4 accesion number | _ |
| GenBank accession number | YP_001966296 |
| Family | TrwB/TraD |
| Length | 512 |
| UniProt ID | Q2QCR0 |
| PDB ID | _ |
| Pfam | TrwB_AAD_bind [PF10412.8], Evalue: 1.40E-101, Aligned region: 110..492 TraG-D_C [PF12696.6], Evalue: 5.50E-22, Aligned region: 349..473 T4SS-DNA_transf [PF02534.13], Evalue: 4.20E-09, Aligned region: 297..488 DUF87 [PF01935.16], Evalue: 5.90E-08, Aligned region: 101..241 |
| Note | Type IV secretion-system coupling protein |
| Protein sequence [Download] | MNERQRSQICLAVMVLVPLFAWFFTAKLMYGIEKQGARDRVFYLAKNTFTLWPLAVALVG GLILAIALCVLIVKLSKTTFAGAHFDKYFRGSQLVSQRELKRLTKEKEEQITIAGVPVPI AAEATHFSVGGATGTGKSTIFREMMYGCMQRKDRMVILDPDGEFLSTFYRKGDKILNPYD ARTEGWNFYNEIRSHYDFERYAKSIIQVSDSNDSEEWNQYGRLLFAEVAKKLYNTTRNPG MREVFRWTNECDSETLQAFVKGTRAVSLFTENERATGSVRFVLSNKLPAHFDMPPGNFSL RQWCEDPNGGNLFITWDENMREALRPLISCWVDTIFTSILGMKSNPKRRIWTFLDELESL QRLPTLGDMLTRGRKKGGCVVSGYQSYTQLESVYGEKLAETMLANHRTMVALAVGRMGTA TAERMSKALSEHEVLRTKEGRSSRWGDWGTRSENEDVKPERIVMSSEIMALNNLEGFLSF PGSIPIARVTIDPINFTRTNPVPGIVPNLEII |
Reference| [1] Bramucci M et al (2006) Genetic organization of a plasmid from an industrial wastewater bioreactor. Appl Microbiol Biotechnol. 71(1):67-74. [PMID:16244860] |
| ID | 139 |
| Plasmid name | pCT14 |
| GenBank accession number | NC_010891.1 |
| Incompatibility group | IncN-like |
| Genome size | 55216 bp |
| Coordinate of oriT [Strand] | 17343..17372 [-] |
| Drug resistance | _ |
| Heavy-metal resistance | mercury resistance |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Pseudomonas sp. CT14 [341029] |
Reference| [1] Bramucci M et al (2006) Genetic organization of a plasmid from an industrial wastewater bioreactor. Appl Microbiol Biotechnol. 71(1):67-74. [PMID:16244860] |