oriTDB
The information of the oriT region
oriTDB accession number 100143
Name oriT_pMG2200  insolico
Sequence Completeness intact
oriT length 322 nt
oriT sequence AGAACAATCAACAACTAATTAGGCAAATTAAGGGGTGCTAAACAACTGCTAGTAGGTGCT AGAGATGTGCTATAAAGGGTGCTAGTTTGGTGCTAGTTACTGCTAAATACGTGCTAGTTT AGGTGCTAGAAACGTGCTATATGGTGCTAAAAAGGTGCTAGTTTGCATGAAGTTACCTGC TAGCCAAGTGCTAGTGGCGTTCGTTTTTGGGTCCCACGGGAAAGCCTTGCACTGCAAGGC GGGTCAGCTTGTCTGACCCCCATTTCCCCTTATGCTCTTCCGAAACACAAAGCGCAATTA AGCGAATACTAGAGAATAAATA
IR (inverted repeat)IR1: [224-231] [233-240] nt  (GCCTTGCA..TGCAAGGC)
IR2: [241-247] [253-259] nt  (GGGTCAG..CTGACCC)
Location of nic site _
Conserved sequence flanking the
  nic site 		_
Note _
L:224-231;R:233-240;L:241-247;R:253-259
Visualization of oriT structure
Reference
[1] Zheng B et al (2009) Isolation of VanB-type Enterococcus faecalis strains from nosocomial infections: first report of the isolation and identification of the pheromone-responsive plasmids pMG2200, Encoding VanB-type vancomycin resistance and a Bac41-type bacteriocin, and pMG2201, encoding erythromycin resistance and cytolysin (Hly/Bac). Antimicrob Agents Chemother. 53(2):735-47. [PMID:19029325]
I. Information of Relaxase
ID 137
Name ORF44_pMG2200 insolico
GenBank accession number YP_002333406
Family MOBP
Length 686 aa
UniProt ID B6ZHK5
PDB ID _
Pfam
Note putative relaxase (plasmid)
Protein sequence [Download] MNKKTAGIILTSAFVMPGTDTFKEYLDYMERSEATRNEHFDQFNAFSPDNSMDSIAAVEG
SNETMFSTYNDYMSNPKKTSALFTNNYDQAPEEVIRYMKEYFEMAQENKSPLWQLVFSFR
NEWLIENNYLDPETNQLKTQHIYQATRLAIAELEKKEGLKGEWTGAVHYNTDNIHVHVGY
VEKNPTREWIFYKHPKEPSKTGWQFKGKFLQKSIKATKRTFVNELMQNKEQLSMLETYVT
HQKKLAESCKEQFTQGKYEQLFTDLLQKLPSNQYFWKYGFAERHKFKPELDKIVDLFMET
DGKETMDEILPRLQKISDDYEEAYGNPRNEPTYLDKQLYGKSGLYSRIGNMILQEAKNYP
ELLAKRKTGLMNESDFEQFNFETEIESEGLIEPFDSLEMTMPQEENAEESMIYDDVLPHE
LLEESHEEVNNIEEQIKVKQSENKMDQALKTLHDYFTEREESKDSRNLKIVDQLKAEKES
SKKEIRGMFLHEVEGNQFAEPVSSFTHETLGRKIILPSKNKAHYISIETSKDKEVDSNLQ
SFSGNNQRAILQQIPTATHVLGEKQWQLNNRQIREDEIENAIKIVTIKKDESGSFVFETK
DVYDISQTDKLPKKERNANYKIEKATEKNGQKRVTKVSNRKPVVSHKKSDYEFKKQLRQL
RKSCERSVQRYLNEKAYQELEQSMNI
Reference
[1] Zheng B et al (2009) Isolation of VanB-type Enterococcus faecalis strains from nosocomial infections: first report of the isolation and identification of the pheromone-responsive plasmids pMG2200, Encoding VanB-type vancomycin resistance and a Bac41-type bacteriocin, and pMG2201, encoding erythromycin resistance and cytolysin (Hly/Bac). Antimicrob Agents Chemother. 53(2):735-47. [PMID:19029325]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 201 _ insolico
Information of T4CP
ID 137
Name VirD4_pMG2200 insolico
SecReT4 accesion number _
GenBank accession number YP_002333362
Family VirD4/TraG
Length 843
UniProt ID B6ZHS5
PDB ID _
Pfam T4SS-DNA_transf [PF02534.13], Evalue: 7.00E-22, Aligned region: 119..655
TraG-D_C [PF12696.6], Evalue: 4.70E-17, Aligned region: 513..633
Note coupling protein (plasmid)
Protein sequence [Download] MKKKEKGKFKKKNYEKAKQNNLRFLADKRFLIPAMIFLFLTEIIVGNWLLQNIFSLLKSN
LFSIQWVEFKETLFYYPLSEYLFAYILLFIASILSTSIIAYRMYTSFRSLEEEHVQGTMD
FESTEGMDKQYPTVEVHPFTKTEDFYDGKPGYPVGRKPKTMKQKAEGTFSLYVDTTDSNT
ITLAGTRQGKGIYFVDTFIDILTRARLIANRASFVMTATKGDEPRKWYNTLKRRGYRIRI
ANVVNPYYSDPIPALAVFNNYYRHYKRLKKESVGLKEKGKASERLNVSIKAEQQLSNAEK
IIKQVAFSYFREVNEGKDGGFWTKACRSLFMSVGLALADQEYEENETSKVNPYTIYNVVN
DMQRRRIKENSHEFLKKYSNDENELQRLLAEYENKSELDVFFGELPRTHPAKRHYDAIFA
SAPAHVTLGNIITHFDGDLEPFLMTLNAKMTAIDDGFDMELVGFDREQPTAVFVMLSDAD
TSNNQLGVMYLEQIYQVLLNRCNLEDDSQCYRDVHFIYEEGGNLGVAISDLARKWTAGLS
RRLFSHLVLQDIQQLSSLYDEDTKDIILGNTGNLAYIRTGSESTNKYISGRLGDRSNYSK
TRHKDPLSIKSTETESTERIDLLASFELERLREGESVILRINKHRDLEGNPIYQYPIYNT
YENDTNLIPFYKFRKLDKVSWEEIPVNNQFMEIELEDLNWKLQPGKIIDQQKQVIPQIPT
YKKPERRKDSNSITPIEQIAKKEVKSVFSTEKYAKEAKFLEDMYGDKLEHLVINVFSVEQ
QKRLIALIQMTGEKEPQSFAELERVSKTGNVRSYISCILSSFGQGAVNMVVQQLKEWRNS
NEF

Reference
[1] Zheng B et al (2009) Isolation of VanB-type Enterococcus faecalis strains from nosocomial infections: first report of the isolation and identification of the pheromone-responsive plasmids pMG2200, Encoding VanB-type vancomycin resistance and a Bac41-type bacteriocin, and pMG2201, encoding erythromycin resistance and cytolysin (Hly/Bac). Antimicrob Agents Chemother. 53(2):735-47. [PMID:19029325]
Information of plasmid
ID 137
Plasmid name pMG2200
GenBank accession number NC_011642.1
Genome size 106527 bp
Coordinate of oriT  [Strand] 43622..43943 [+]
Drug resistance experimental vancomycin resistance
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Enterococcus faecalis [1351]
Reference
[1] Zheng B et al (2009) Isolation of VanB-type Enterococcus faecalis strains from nosocomial infections: first report of the isolation and identification of the pheromone-responsive plasmids pMG2200, Encoding VanB-type vancomycin resistance and a Bac41-type bacteriocin, and pMG2201, encoding erythromycin resistance and cytolysin (Hly/Bac). Antimicrob Agents Chemother. 53(2):735-47. [PMID:19029325]

Microbial Bioinformatics Group in MML, SJTU