oriTDB
The information of the oriT region
oriTDB accession number 100140
Name oriT2_pMAS2027  experimental
Sequence Completeness incomplete
oriT length 19 nt
oriT sequence TTGCCCTATCCTGCATCGC
IR (inverted repeat)_
Location of nic site [13-14] nt
Conserved sequence flanking the
  nic site
TATCCTG|C
Note _
D:13-14
Visualization of oriT structure (The oriT was characterized experimentally)
Reference
[1] Ong CL et al (2009) Conjugative plasmid transfer and adhesion dynamics in an Escherichia coli biofilm. Appl Environ Microbiol. 75(21):6783-91. [PMID:19717626]
I. Information of Relaxase
ID 134
Name TaxC_pMAS2027 insolico
GenBank accession number YP_003292208
Family MOBP
Length 390 aa
UniProt ID D0QMQ2
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 5.40E-10, Aligned region: 66..309
Note Relaxase
Protein sequence [Download] MIMGVYVDKEYRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINRKGASKEVVVKISG
GAVTRQGIRNSIDYMSRESELPVMSESGRVWTGDEILEAKDHMIDRANDPQHVMNDKGKE
NKKITQNIVFSPPVSAKVKPEDLLESVRKTMQKKYPNHRFVLGYHCDKKEHPHVHVVFRI
RDNDGKRADIRKKDLREIRTGFCEELKLKGYDVKATHKQQHGLNQSVKDAHNTAPKRQKG
VYEVVDVGYDHYQNDKTKSKQHFIKLKTLNKGVEKTYWGADFGDLCSRESVKAGDLVRLK
KLGQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGVKGVDRTPSASKELVLNSPDMLLKQ
QQRMAQFTQQKASTLQSEQKLKTGIKFWSL
Reference
[1] Ong CL et al (2009) Conjugative plasmid transfer and adhesion dynamics in an Escherichia coli biofilm. Appl Environ Microbiol. 75(21):6783-91. [PMID:19717626]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 197 Ddp1_pMAS2027 insolico YP_003292209 204 aa similar to TaxA from R6K
Information of T4CP
ID 134
Name TaxB_pMAS2027 insolico
SecReT4 accesion number _
GenBank accession number YP_003292195
Family VirD4/TraG
Length 613
UniProt ID D0QMN9
PDB ID _
Pfam T4SS-DNA_transf [PF02534.13], Evalue: 1.70E-143, Aligned region: 103..580
TraG-D_C [PF12696.6], Evalue: 2.90E-24, Aligned region: 417..537
TrwB_AAD_bind [PF10412.8], Evalue: 1.70E-16, Aligned region: 309..553
Note bacterial conjugative protein involved in type IV sectretion
Protein sequence [Download] MIMSLKLPDKGQWAFIGLVMCLVTYYTGSVAVYFLNGKTPLYIWKNFDSMLLWRIITESN
IRIDIRLTAIPSLLSGMVSSLIVPVFIIWQLNKTDVALYGDAKFASDNDLRKSKLLKWEK
ENDTDILVGAYKGKYLWYTAPDFVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELW
KITSKVREILLGNKVYLLDPFNSKTHQFNPLFYIDLKAESGAKDLLKLIEILFPSYGMTG
AEAHFNNLAGQYWTGLAKLLHFFINYEPSWLNEFGLKPVFSIGSVVDLYSNIDRELILSK
REELEGTNGLDENALYHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYLPTVRKCTD
GNDFDLRQLRREDITVYVGVNAEDISLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLM
FLDEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIY
AVSEEDDAAKISEKLGYITTTSKSTSKSRGRSTSQGESESEARRALVLPQELGTLDFKEE
FIILKGENPVKAEKALYYLDPYFMDRLMKVSPKLASLTMELNKTKKIFGVKGLKYPSKEK
MLSVGELESEVLL

Reference
[1] Ong CL et al (2009) Conjugative plasmid transfer and adhesion dynamics in an Escherichia coli biofilm. Appl Environ Microbiol. 75(21):6783-91. [PMID:19717626]
Information of plasmid
ID 134
Plasmid name pMAS2027
GenBank accession number NC_013503.1
Incompatibility group IncX1
Genome size 42644 bp
Coordinate of oriT  [Strand] 2060..2078 [-]; 38362..38380 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor experimental Plasmid pMAS2027 encodestwo important virulence factors: type 3 fimbriae and a type IV secretion (T4S) system
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli MS2027 [562]
Reference
[1] Ong CL et al (2009) Conjugative plasmid transfer and adhesion dynamics in an Escherichia coli biofilm. Appl Environ Microbiol. 75(21):6783-91. [PMID:19717626]