 oriTDB accession number | 100136 |
| Name | oriT_pLAC1  |
Sequence Completeness  | incomplete |
| oriT length | 32 nt |
| oriT sequence | AACATAAAGTATAGTGAGTTATACTTTACTTG |
| IR (inverted repeat) | [5-13] [20-28] nt (TAAAGTATA..TATACTTTA) |
| Location of nic site | [17-18] nt |
| Conserved sequence flanking the nic site | TAGTGA|GTTAT |
| Note | pMV158 superfamily; nick site (TGA′G) |
Visualization of oriT structure Reference| [1] Asteri IA et al (2010) Characterization of a small cryptic plasmid from endophytic Pantoea agglomerans and its use in the construction of an expression vector. Int J Food Microbiol. 141(3):222-8. [PMID:20538362] |
| ID | 131 |
| Name | Mob_pLAC1 |
| GenBank accession number | YP_003650630 |
| Family | MOBV |
| Length | 361 aa |
| UniProt ID | D6CHH5 |
| PDB ID | _ |
| Pfam | Mob_Pre [PF01076.18], Evalue: 1.20E-77, Aligned region: 1..194 |
| Note | clade A of the pMV158-superfamily of relaxases |
| Protein sequence [Download] | MSFAVARMTKLKADNLVGIGNHDQRKTTNHSNEDIDVSRSHLNYDLVAGRTDNFKTDIEA YINENKASKRAVRKDAVLVNEWIITSDKDFFEQLDEAETRKYFETAKQYFADNYGDENIR YAVVHMDEKTPHMHMGIVPFDDGKKLSAKRIFNREALQRIQEELPQYLKENGFDVQRGNK NKERKNLSVPEYKAMREDLKKIETEKQETQAKLADTKKQLDRIKPRDTKKIASKPTLMNK NKVTVDKSDLADLEQRAVTSDAYNFEKIHLEVENHSLRNDLGEAKGQNYELKKENERLQK LVGTLQGIIRNVDGFLHKKLGINLPEKWLERAGLKEPSKKAPESSQELGKHKSDELGGPH L |
Reference| [1] Asteri IA et al (2010) Characterization of a small cryptic plasmid from endophytic Pantoea agglomerans and its use in the construction of an expression vector. Int J Food Microbiol. 141(3):222-8. [PMID:20538362] |
| # | ID | Name | GenBank | Length | Note |
| 1 | 194 | _ |
| ID | 131 |
| Plasmid name | pLAC1 |
| GenBank accession number | NC_014164.1 |
| Genome size | 3478 bp |
| Coordinate of oriT [Strand] | |
| Drug resistance | _ |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Lactobacillus acidipiscis ACA-DC 1533 [89059] |
Reference