oriTDB
The information of the oriT region
oriTDB accession number 100121
Name oriT_2s-pEI2  insolico
Sequence Completeness incomplete
oriT length 22 nt
oriT sequence GCACTCTGTGTATGCTGGCTTA
IR (inverted repeat)_
Location of nic site [18-19] nt
Conserved sequence flanking the
  nic site
CTGG|CTTA
Note ColE1 superfamily
D:18-19
Visualization of oriT structure
Reference
[1] Rogge ML et al (2013) Comparison of Vietnamese and US isolates of Edwardsiella ictaluri. Dis Aquat Organ. 106(1):17-29. [PMID:24062549]
I. Information of Relaxase
ID 116
Name MobA_2s-pEI2 insolico
GenBank accession number YP_007459738
Family MOBP
Length 357 aa
UniProt ID M1L0K9
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 3.70E-06, Aligned region: 1..173
Note relaxase
Protein sequence [Download] MTSFEKALLPGLDADQYACLWVEHKDKGRLELNFVVPNIELTSGKRLQPYFDRADRPRID
AWKTGMNASLSLLDPNDPMKKRELVTPRNLPPIKQEAARVITDSLLRLAELRDDKRSQYV
VQSLEHAGFTVCRQTKSSISIADPDGGRNIRLKGMIYEQNFRFGPELRGEIEAASERYRK
TSSDRTRDARNVYQRCFRQKRDDNQRRYKRPESSYTGLTVKNMALDGPQPDRHPDSLLRR
DLATRRDDHPELADHQRAERHAPAARSQGWENSNEHMRRQETPVREDRSGCRHVWGHPEG
RLSVEDTGGVLSDDRARTAAFERIRRIADAARRATQRIQNLINPNLGNGAAFSKQGS
Reference
[1] Rogge ML et al (2013) Comparison of Vietnamese and US isolates of Edwardsiella ictaluri. Dis Aquat Organ. 106(1):17-29. [PMID:24062549]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 174 MobC_2s-pEI2 insolico YP_007459741 111 aa Bacterial mobilization protein (MobC)
 N/A
Information of plasmid
ID 116
Plasmid name 2s-pEI2
GenBank accession number NC_020295.1
Genome size 9103 bp
Coordinate of oriT  [Strand] 1867..1888 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Edwardsiella ictaluri strain 2s [67780]
Reference
 N/A