oriTDB
The information of the oriT region
oriTDB accession number 100112
Name oriT_pMdT1  insolico
Sequence Completeness core
oriT length 64 nt
oriT sequence AGGTTTCGGGGCGCAGCCCTGAACCAGTCACCTAGCGCTAGCGGAGTGTATACTGGCTTA GTAT
IR (inverted repeat)IR1: [5-12] [16-23] nt  (TTCGGGGC..GCCCTGAA)
IR2: [32-36] [37-41] nt  (CTAGC..GCTAG)
Location of nic site [56-57]nt
Conserved sequence flanking the
  nic site 		CTGG|CTTA
Note ColE1 superfamily
L:5-12;R:16-23;L:32-36;R:37-41;D:56-57
Visualization of oriT structure
Reference
[1] de Toro M et al (2013) pMdT1, a small ColE1-like plasmid mobilizing a new variant of the aac(6')-Ib-cr gene in Salmonella enterica serovar Typhimurium. J Antimicrob Chemother. 68(6):1277-80. [PMID:23361643]
I. Information of Relaxase
ID 107
Name MobA_pMdT1 insolico
GenBank accession number YP_006957893
Family MOBP
Length 516 aa
UniProt ID K4I4Y4
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 3.70E-17, Aligned region: 58..264
Note mobilization protein MobA
Protein sequence [Download] MPDSSGCGMPCWKRVQTMIVKFHPRGRGGGAGPVDYLLGKDRQRDGATVLQGKPEEVREL
IDASPYAKKYTSGVLSFAEKDLPPGQREKLMASFERVLMPGLDKDQYSVLWVEHQDKGRL
ELNFLIPNMELLTGRRLQPYYDRADRPRIDAWQTIVNGRLGLHDPNAPENRRALVTPSAL
PKTKQEAAQAITRGLLALASSGELKTRQDVTEALESAGFEVVRTTKSSISIADPDGGRNI
RLKGAIYEQSFNAGKGLRAEIESAAAEYRRDAESRIQRAREVCQSGTERKREENQRRHPR
PRPEAVLSHEPAHERHGAYGQPDVADHRPGVYTADSIGRKHSMVAGAADTRQLHDHPGAE
EHAGHAVREEQRRAALDLRRAETPLREGEPGSGTVRRGFELDDTGGEIAHDGAGKTVAER
IRAATAGLLEKAGRVGERLRGMADDVWSYATGERGAERARHGLEQAGAEFERAAAPVVTR
LNVIEAHREQERAAQHQKALELERSQRQRTYNGPSL
Reference
[1] de Toro M et al (2013) pMdT1, a small ColE1-like plasmid mobilizing a new variant of the aac(6')-Ib-cr gene in Salmonella enterica serovar Typhimurium. J Antimicrob Chemother. 68(6):1277-80. [PMID:23361643]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 162 MobB_pMdT1 insolico YP_006957895 161 aa MobB mobilisation protein
2 163 MobC_pMdT1 insolico YP_006957896 107 aa MobC mobilisation protein
 N/A
Information of plasmid
ID 107
Plasmid name pMdT1
GenBank accession number NC_019133.1
Genome size 5931 bp
Coordinate of oriT  [Strand] 1978..2041 [+]
Drug resistance experimental resistance to tobramycin and kanamycin, and also decreased susceptibility to ciprofloxacin and norfloxacin in the transformant strain
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Salmonella enterica serovar Typhimurium Se20 [90371]
Reference
[1] de Toro M et al (2013) pMdT1, a small ColE1-like plasmid mobilizing a new variant of the aac(6')-Ib-cr gene in Salmonella enterica serovar Typhimurium. J Antimicrob Chemother. 68(6):1277-80. [PMID:23361643]

Microbial Bioinformatics Group in MML, SJTU