oriTDB
The information of the oriT region
oriTDB accession number 100103
Name oriT_pAsa3  insolico
Sequence Completeness incomplete
oriT length 19 nt
oriT sequence TACGGGTAGGCTGGCTTAA
IR (inverted repeat)_
Location of nic site [14-15] nt
Conserved sequence flanking the
  nic site
CTGG|CTTA
Note ColE2-type
D:14-15
Visualization of oriT structure
Reference
[1] Boyd J et al (2003) Three small, cryptic plasmids from Aeromonas salmonicida subsp. salmonicida A449. Plasmid. 50(2):131-44. [PMID:12932739]
I. Information of Relaxase
ID 98
Name MobA_pAsa3 insolico
GenBank accession number AAP69902
Family MOBP
Length 555 aa
UniProt ID Q7X2D9
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 1.60E-08, Aligned region: 35..254
Note mobilization relaxase
Protein sequence [Download] MHIKFLAHGTGSGAHASAYLMGPHDHTGEPRAGVAVLRGDPVLFAAVADSLPFQQRYSSA
VISWAAEEAPRPDEISAVLDDFEALAFAGLAREDVHLTAVEHAEADGGVHVHILIPRVHL
GSGKSFNPAPPGQRKDFDAVRDKHNHEKGWARPDDPLRARLLRPDFEAYKAVTDHTAIKK
QLTDHLIGAVAQGVVSNADELRAYLQEGLGCEITRTGKDYTSVKPEGYPKAIRLKGELYG
ESWTAQSTLEREAQAAARAGIGRGGEVSASAARDAQERLAGAHQRRAEYNRDRYQGRDRG
LAPADLRREQTLENSLEGGQGRSRHAETGLQRLVQSRAAGDFLRGGVTDGKLVWHELDMG
ADRTRHGDVAEFAARDQQRPEHVGRVAEGGELLQGGGWKTLHHRPQDWGAVQDRGGQDGR
GGALNDRAGAGTVGEAGADRTAARATNRGLEAAIAAASALFERVAKGVQRCAQELREAMQ
RPARGDPDVAERGNALEQGYQRSARELEQQCQRLEQGAGELAERAGLINEQAKELKESKE
LELSLEYERYRGLEL
Reference
[1] Boyd J et al (2003) Three small, cryptic plasmids from Aeromonas salmonicida subsp. salmonicida A449. Plasmid. 50(2):131-44. [PMID:12932739]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 141 MobB_pAsa3 insolico AAP69903 185 aa mobilization protein
2 142 MobC_pAsa3 insolico AAP69901 136 aa mobilization protein
3 143 MobD_pAsa3 insolico AAP69904 102 aa mobilization protein
 N/A
Information of plasmid
ID 98
Plasmid name pAsa3
GenBank accession number AY301065.1
Genome size 5616 bp
Coordinate of oriT  [Strand] 3437..3455 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Aeromonas salmonicida A449 [382245]
Reference
 N/A