oriTDB
The information of the oriT region
oriTDB accession number 100099
Name oriT_pKPC-LKEc  insolico
Sequence Completeness intact
oriT length 83 nt
oriT sequence GGGGTGTCGGGGCGAAGCCCTGACCAGATGGTAATTGTAATAGCGTCGCGTGTGACGGTA TTACAATTACACATCCTGTCCCG
IR (inverted repeat)IR1: [6-13] [17-24] nt  (GTCGGGGC..GCCCTGAC)
IR2: [31-47] [54-70] nt  (GTAATTGTAATAGCGTC..GACGGTATTACAATTAC)
Location of nic site [78-79] nt
Conserved sequence flanking the
  nic site 		CATCCTG|T
Note _
L:6-13;R:17-24;L:31-47;R:54-70;D:78-79
Visualization of oriT structure
Reference
[1] Chen YT et al (2014) KPC-2-encoding plasmids from Escherichia coli and Klebsiella pneumoniae in Taiwan. J Antimicrob Chemother. 69(3):628-31. [PMID:24123430]
I. Information of Relaxase
ID 94
Name NikB_pKPC-LKEc insolico
GenBank accession number AGS77392
Family MOBP
Length 899 aa
UniProt ID U5KKC0
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 1.70E-62, Aligned region: 47..315
Note Relaxase/Mobilisation nuclease
Protein sequence [Download] MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELNLSSSSQAEQPHRSRFSRLVDYATRL
RNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIAQQAHYAKDNTDPVFHY
ILSWQAHESPRPEQIYDSVRHTLKSLGLGEHQYVSAVHTDTDNLHVHVAVNRVHPVTGYL
NCLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRIVRKTAVERDRQNAWTRGKKQTF
REYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGKFLVMDGWDRNREGVQLDSFG
PSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETESLQQYACRHL
GERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKREL
FSDKESLWGYSISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHE
QTPVKASSIHPDLTLGRAEPQAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDP
MLRRQRREARAEARADLRARYLAWREQWRKPDLRYGERCREIHQACRLRKSHIRAQYDDP
ALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVS
QLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGE
FVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG
Reference
[1] Chen YT et al (2014) KPC-2-encoding plasmids from Escherichia coli and Klebsiella pneumoniae in Taiwan. J Antimicrob Chemother. 69(3):628-31. [PMID:24123430]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 135 NikA_pKPC-LKEc insolico AGS77391 110 aa conjugal transfer relaxosome component
 N/A
Information of plasmid
ID 94
Plasmid name pKPC-LKEc
GenBank accession number KC788405.1
Incompatibility group IncI
Genome size 145401 bp
Coordinate of oriT  [Strand] 30139..30221 [-]
Drug resistance insolico blaKPC-2 (encoding beta-lactamase KPC-2)
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli LK-NARMP [562]
Reference
[1] Chen YT et al (2014) KPC-2-encoding plasmids from Escherichia coli and Klebsiella pneumoniae in Taiwan. J Antimicrob Chemother. 69(3):628-31. [PMID:24123430]

Microbial Bioinformatics Group in MML, SJTU