oriTDB
The information of the oriT region
oriTDB accession number 100084
Name oriT_pNM5  insolico
Sequence Completeness core
oriT length 35 nt
oriT sequence CCAGAAGAATTCTGGTATACCGTGTTATACCAAAT
IR (inverted repeat)[1-6] [10-15] nt  (CCAGAA..TTCTGG)
Location of nic site [24-25] nt
Conserved sequence flanking the
  nic site
TACCGTG|TTAT
Note _
L:1-6;R:10-15;D:24-25
Visualization of oriT structure
Reference
[1] Alippi AM et al (2014) Tetracycline-resistance encoding plasmids from Paenibacillus larvae, the causal agent of American foulbrood disease, isolated from commercial honeys. Int Microbiol. 17(1):49-61. [PMID:25296446]
I. Information of Relaxase
ID 79
Name Mob_pNM5 insolico
GenBank accession number AFU63384
Family MOBV
Length 444 aa
UniProt ID K4IDV6
PDB ID _
Pfam Mob_Pre [PF01076.18], Evalue: 1.40E-32, Aligned region: 8..187
Note nicks plasmid at oriT to initiate conjugation
Protein sequence [Download] MLFLTENEVSEIAHVQKYTKANMQGLSIHLDRKTENHSNEDIDIERSHLNYDLCEKEGDT
LSRMNERLDEVYCMDRKDVKACCSWVVTLPENLKDAPDEHQRKFFEETYGFLADRYGGEK
NVLSANVHNDETTPHMHFAFIPVVYDPKKEREKVSAKLVINRNELKVFHKDLDVHLKESI
PEIYQDGILNDKTIGLDDIESIKKHSKEIEAKKADMSKELEEFSRKEKEEMVKDLATFKK
PDEIIKRIDSTSKESLLGGKTNLITKDYKQLIKVAKSAIKEKQNLYNYKKVSSEKIGDLE
ATVRFADRKIKNLEGKNENLEKEVAGLQEHRINEIVFKSMLQDDNRDLNISQTEIDGRLI
LFNLENGHEPLDREQGERWSSTLEENKRLKTIPQSRLQRGLDKLKQFIEKFLVRGFSMDS
VLQKVEQEKSRKPKNKKKSHDLEL
Reference
[1] Alippi AM et al (2014) Tetracycline-resistance encoding plasmids from Paenibacillus larvae, the causal agent of American foulbrood disease, isolated from commercial honeys. Int Microbiol. 17(1):49-61. [PMID:25296446]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 115 _ insolico
 N/A
Information of plasmid
ID 79
Plasmid name pNM5
GenBank accession number JX863070.1
Genome size 5109 bp
Coordinate of oriT  [Strand] 3401..3435 [+]
Drug resistance insolico resistance to tetracycline
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Uncultured marine bacterium [56765]
Reference
[1] Alippi AM et al (2014) Tetracycline-resistance encoding plasmids from Paenibacillus larvae, the causal agent of American foulbrood disease, isolated from commercial honeys. Int Microbiol. 17(1):49-61. [PMID:25296446]