oriTDB
The information of the oriT region
oriTDB accession number 100083
Name oriT_pMAB01  experimental
Sequence Completeness intact
oriT length 304 nt
oriT sequence TGCCTCGCAGAGCAGGATGACCCGTTGAGCGCCCCCGGCGCGAATAAGGGACAGTGAAGA TAGATAACCGGCTCGCCGGTTAGCTAACTTCACACATCCTGCCCGCCTTACGGCGTTAAT AACACCAAGGAAAGTCTACACCAGCCATTACGATTTATCCGCAACTATCGCGCTATCAGG CCGCAAAAGCAGCAACGGATATAGCGAAAACCGCCACAATGGCCCATAATGCCGCTATCG AAGCGTGCCAATGCACGCCGATAGCGGACTTTTTGCGTTTCCGTAGCGCCGCTTAGTAGC GTTA
IR (inverted repeat)[55-72] [75-92] nt  (TGAAGATAGATAACCGGC..GCCGGTTAGCTAACTTCA)
Location of nic site [101-102] nt
Conserved sequence flanking the
  nic site 		CATCCTG|C
Note similar to oriT of the IncP-1beta multiresistance plasmid pB10
L:55-72;R:75-92;D:101-102
Visualization of oriT structure (The oriT was characterized experimentally)
Reference
[1] Leão SC et al (2013) The detection and sequencing of a broad-host-range conjugative IncP-1β plasmid in an epidemic strain of Mycobacterium abscessus subsp. Bolletii. PLoS One. 8(4):e60746. [PMID:23565273]
I. Information of Relaxase
ID 78
Name TraI_pMAB01 insolico
GenBank accession number AGJ01256
Family MOBP
Length 746 aa
UniProt ID _
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 9.90E-64, Aligned region: 12..252
Note conjugal transfer relaxase TraI; Provisional
Protein sequence [Download] MIAKHVPMRSLGKSDFAGLANYITDAQSKDHRLGHVQATNCEAGSIQDAITEVLATQHTN
TRAKGDKTYHLIVSFRAGEQPSADTLRAIEERICVGLGYGEHQRISAVHNDTDNLHIHIA
INKIHPTRHTMHEPYYPHRALAELCTALERDYGLERDNHEPRKRGAEGRAADMERHAGVE
SLVGWIKRECLDEIKGAQSWQELHQVMRDNGMELRVRANGLVFEAGDGTMVKASTVARDL
SKPSLEARLGPFEASPERQAQTTAKRQYRKDPIRLRVNTVELYAKYKAEQQSLTTARAQA
LERARHRKDRLIEAAKRSNRLRRATIKVVGEGRANKKLLYAQASKALRSELQAINKQFQQ
ERTALYAEHSRRTWADWLKKEAQHGGADALAALRAREAAQGLKGNTIRGEGQAKPGHAPA
VDNITKKGTIIFRAGMSAVRDDGDRLQVSREATREGLQEALRLAMQRYGNRITVNGTVEF
KAQMIRAAVDSQLPITFTDPALESRRQALLNKENTHERTERPEHRGRTGRGAGGPGQRPA
ADQHATGAAAVARAGDGRPAAGRGDRADAGLHAATVHRKPDVGRLGRKPPPQSQHRLRAL
SELGVVRIAGGSEVLLPRDVPRHVEQQGTQPDHALRRGISRPGTGVGQTPPGVAAADKYI
AEREAKRLKGFDIPKHSRYTAGDGALTFQGTRTIEGQALALLKRGDEVMVMPIDQATARR
LTRIAVGDAVSITAKGSIKTSKGRSR
Reference
[1] Leão SC et al (2013) The detection and sequencing of a broad-host-range conjugative IncP-1β plasmid in an epidemic strain of Mycobacterium abscessus subsp. Bolletii. PLoS One. 8(4):e60746. [PMID:23565273]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 112 TraH_pMAB01 insolico AGJ01257 130 aa _
2 113 TraJ_pMAB01 insolico AGJ01258 124 aa conjugal transfer relaxosome component TraJ
3 114 TraK_pMAB01 insolico AGJ01259 132 aa _
Information of T4CP
ID 78
Name TraG_pMAB01 insolico
SecReT4 accesion number _
GenBank accession number AGJ01255
Family VirD4/TraG
Length 637
UniProt ID _
PDB ID _
Pfam T4SS-DNA_transf [PF02534.13], Evalue: 2.70E-162, Aligned region: 121..624
TraG-D_C [PF12696.6], Evalue: 5.50E-32, Aligned region: 444..569
TrwB_AAD_bind [PF10412.8], Evalue: 5.10E-22, Aligned region: 187..586
Note onjugal transfer coupling protein TraG
Protein sequence [Download] MKIKMNNAVGPQVRTAKPKPSKLLPVLGAASMVGGLQAATQFFAHTFAYHATLGPNVGHV
YAPWSILHWTYKWYSQYPDEIMKAGSMGMLVSTVGLLGVAVAKVVTSNSSKANEYLHGSA
RWAEKKDIQAAGLLPRERNVLEIVTGKAAPTATGVYVGGWQDKDGNFFYLRHSGPEHVLT
YAPTRSGKGVGLVVPTLLSWGASSVITDLKGELWALTAGWRQKHAKNKVLRFEPASTSGG
VCWNPLDEIRLGTEYEVGDVQNLATLIVDPDGKGLDSHWQKTAFALLVGVILHALYKAKD
DGGTATLPSVDAMLADPNRDIGELWMEMATYGHVDGQNHHAIGSAARDMMDRPEEEAGSV
LSTAKSYLALYRDPVVARNVSRSDFRIKQLMHEDDPVSLYIVTQPNDKARLRPLVRVMVN
MIVRLLADKMDFEGGRPVAHYKHRLLMMLDEFPSLGKLEIMQESLAFVAGYGIKCYLICQ
DINQLKSRETGYGHDESITSNCHVQNAYPPNRVETAEHLSRLTGQTTVVKEQITTSGRRT
AAMLGQVSRTYQEVQRPLLTPDECLRMPGPKKNAQGEIEEAGDMVIYVAGYPAIYGKQPL
YFKDPVFSARAAIPAPKVSDRLRAVAQAETEGEGITI

Reference
[1] Leão SC et al (2013) The detection and sequencing of a broad-host-range conjugative IncP-1β plasmid in an epidemic strain of Mycobacterium abscessus subsp. Bolletii. PLoS One. 8(4):e60746. [PMID:23565273]
Information of plasmid
ID 78
Plasmid name pMAB01
GenBank accession number CP003376.1
Incompatibility group IncP1
Genome size 56267 bp
Coordinate of oriT  [Strand] 32885..33188 [+]
Drug resistance insolico streptomycin resistance genes (strA strB); aminoglycoside 6鈥?N-acetyltransferase gene (aac(6鈥?-Ib)
Heavy-metal resistance insolico mercury resistance
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Mycobacterium abscessus subsp. bolletii INCQS 00594 [1087469]
Reference
[1] Leão SC et al (2013) The detection and sequencing of a broad-host-range conjugative IncP-1β plasmid in an epidemic strain of Mycobacterium abscessus subsp. Bolletii. PLoS One. 8(4):e60746. [PMID:23565273]

Microbial Bioinformatics Group in MML, SJTU