 oriTDB accession number | 100079 |
| Name | oriT_pRA2  |
Sequence Completeness  | intact |
| oriT length | 394 nt |
| oriT sequence | GCAAACCCCTAATGATGGCGGTGGGACACGCCGCAATGTTGGCCGCTGGCACGGCGCGGG GTCTCGGGGCGAAGCCCTGAGCAGGGGAGGAAAGGCCCAGCAGAACGAGTGCAACGAGTG ACGCGGGCCGGTGGCACACTGCCGCTTTAGCGGCGGTGGGACATTCCCTACCCTGTCCCC TAATATGAAACCGCGTCCCGTTCAAACTTGTCTGCATCACTCACGCCCATTGAAAACCCA CAAAAACCGGCACAGCACGATAAACCCGTGTATATCACAAACGCAGGTACTTGACCACAT CCGCATAATGCACGATGATTGCAAGGCCAATGATTGCGCCAGCTTCAAAGGCAAACATCA CCACACTGTTACCACAATCTAGCGAGGGCCAAAG |
| IR (inverted repeat) | [132-146] [149-163] nt (TGGCACACTGCCGCT..AGCGGCGGTGGGACA) |
| Location of nic site | [175-176] nt |
| Conserved sequence flanking the nic site | TACCCTG|T |
| Note | |
Visualization of oriT structure Reference| [1] van Zyl LJ et al (2003) Analysis of the mobilization region of the broad-host-range IncQ-like plasmid pTC-F14 and its ability to interact with a related plasmid, pTF-FC2. J Bacteriol. 185(20):6104-11. [PMID:14526022] |
| [2] Dam B et al (2009) Conjugative Type 4 secretion system of a novel large plasmid from the chemoautotroph Tetrathiobacter kashmirensis and construction of shuttle vectors for Alcaligenaceae. Appl Environ Microbiol. 75(13):4362-73. [PMID:19411426] |
| ID | 74 |
| Name | MobA_pRA2 |
| GenBank accession number | YP_025347 |
| Family | MOBP |
| Length | 854 aa |
| UniProt ID | Q9XAW2 |
| PDB ID | _ |
| Pfam | Relaxase [PF03432.13], Evalue: 1.70E-80, Aligned region: 12..285 |
| Note | relaxase |
| Protein sequence [Download] | MLAKVPPKRADGKTSFKSLAKYACERDHIDPETGAVERRECSTETNCLDKDTAWREMKAV SDMNGRVKDPVYHFTVSWPAHEKPTDAQVFEAGREGMKSLGMEGHQYLAAVHRDTDNVHG HFMVNRVNPETYKAVYPDRDYFKLDKTMREMELRQGWSHDKGPYAVHERNGQKVVDWAKD EPSHRQPDREKLPGKARQMEVMTGNESLTTYAQGQPKKDALEALNKGGGWHELHEALGRH GLEIRQKGQGFAIHSKTDPEQTPIKASTMAQELGGGKLTKRIGPYQEPRPEIQQEKAKRE YSDQRPKADPKRDPNAREEKRDERAAERRELRDRYTAFTEEWKTAKAPARAAMYEDQKQR RQALTEKHKSQRETIRTSGLSTEQKKALYSVAAFDTAAKRAELNETIKGEREAFRQERPQ SFREWTADRAQEGDRAAMRQVRGWAYQDKRTAKDMQQADAKAERGPHLGTTDTEQHDPAK PRRVTDRVSWAVDRKTGAVDYKVDDRDAFRDSGRRLDYTEQGRKDKDSIEAGLLLAREKF AGKALKVTGPDDFRERVLQTAIERKLDVRFSDKELEQRREAGLRQQAERERPDWSKMRGV ASTGQQAEQRPQQPTQAQQPERRPTAPEQAKEAAQAQPAQQAAPRMTDDEARAVLNRKPP AEPNQELVEINAINAHADGYRQQLDKQFREKHGERPDPERAGGWIARFAAKSKAEAWDKE HAQIDRQVEQRKEHLRSDAPEARQFRAQAWAQAQQQYGAQHTAWEQARKQATAHFEPNRE GEPNMANNEQERKQQQQTQQQAQQKQAQEKQMAERAAREAQARQLAEKQKQQKEKQEKEK EQARMMNQQMDIDR |
Reference| [1] Francia MV et al (2004) A classification scheme for mobilization regions of bacterial plasmids. FEMS Microbiol Rev. 28(1):79-100. [PMID:14975531] |
| # | ID | Name | GenBank | Length | Note |
| 1 | 107 | MobB_pRA2 | YP_025348 | 104 | _ |
| 2 | 108 | MobC_pRA2 | YP_025349 | 116 | _ |
| ID | 74 |
| Plasmid name | pRA2 |
| GenBank accession number | NC_005909.1 |
| Incompatibility group | IncQ2 |
| Genome size | 32743 bp |
| Coordinate of oriT [Strand] | 27593..27986 [+] |
| Drug resistance | _ |
| Heavy-metal resistance | mercury resistance |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Pseudomonas alcaligenes [43263] |
Reference| [1] Yeo CC et al (1998) Tn5563, a transposon encoding putative mercuric ion transport proteins located on plasmid pRA2 of Pseudomonas alcaligenes. FEMS Microbiol Lett. 165(2):253-60. [PMID:9742696] |