 oriTDB accession number | 100075 |
| Name | oriT1_pOLA52  |
Sequence Completeness  | incomplete |
| oriT length | 26 nt |
| oriT sequence | GGCGTTTGCCCTATCCTGCATCGCAG |
| IR (inverted repeat) | _ |
| Location of nic site | [18-19] nt |
| Conserved sequence flanking the nic site | TATCCTG|C |
| Note | oriT_pOLA52 (30771..30796) |
Visualization of oriT structure Reference| [1] Norman A et al (2008) Nucleotide sequence of pOLA52: a conjugative IncX1 plasmid from Escherichia coli which enables biofilm formation and multidrug efflux. Plasmid. 60(1):59-74. [PMID:18440636] |
| ID | 71 |
| Name | TaxC_pOLA52 (IncX1) |
| GenBank accession number | YP_001693207 |
| Family | MOBP |
| Length | 388 aa |
| UniProt ID | B0ZDZ0 |
| PDB ID | _ |
| Pfam | Relaxase [PF03432.13], Evalue: 6.30E-10, Aligned region: 64..306 |
| Note | relaxase |
| Protein sequence [Download] | MGVYVDKEHRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINRKGASKEVVVKISGGA VTRQGIRNSIDYMSRESELPVMSESGRVWTGDEILEAKEHMIDRANDPQHVMNDKGKENK KITQNIVFSPPVSAKVKPEDLLESVRKTMQKKYPNHRFVLGYHCDKKEHPHVHVVFRIRD NDGKRADIRKKDLREIRTGFCEELKLKGYDVKATHKQQHGLNQSVKDAHNTAPKRQKGVY EVVDIGYDHYQNDKTKSKQHFIKLKTLNKGVEKTYWGADFGDLCSRESVKAGDLVRLKKL GQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGVKGVDRTPSASKELVLNSPDMLLKQQQ RMAQFTQQKASTLQSEQKLKTGIKFFNI |
Reference| [1] Norman A et al (2008) Nucleotide sequence of pOLA52: a conjugative IncX1 plasmid from Escherichia coli which enables biofilm formation and multidrug efflux. Plasmid. 60(1):59-74. [PMID:18440636] |
| # | ID | Name | GenBank | Length | Note |
| 1 | 103 | TaxA_pOLA52 | YP_001693208 | 181 aa | auxilary protein; similar to TaxA from R6K |
| ID | 71 |
| Name | TaxB_pOLA52 |
| SecReT4 accesion number | _ |
| GenBank accession number | YP_001693193 |
| Family | VirD4/TraG |
| Length | 611 |
| UniProt ID | B0ZDX6 |
| PDB ID | _ |
| Pfam | T4SS-DNA_transf [PF02534.13], Evalue: 3.50E-143, Aligned region: 101..578 TraG-D_C [PF12696.6], Evalue: 2.90E-24, Aligned region: 415..535 TrwB_AAD_bind [PF10412.8], Evalue: 1.20E-16, Aligned region: 307..551 |
| Note | putative T4SS coupling protein; similar to TaxB from R6K |
| Protein sequence [Download] | MSLKLPDKGQWAFIGLVMCLVTYYTGSVAVYFLNGKTPLYIWKNFDSMLLWRIITESNIR TDIRLTAIPSLLSGMVSSLIVPVFIIWQLNKTDVALYGDAKFASDNDLRKSKLLKWEKEN DTDILVGAYKGKYLWYTAPDFVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKI TSKVREILLGNKVYLLDPFNSKTHQFNPLFYIDLKAESGAKDLLKLIEILFPSYGMTGAE AHFNNLAGQYWTGLAKLLHFFINYEPSWLNEFGLKPVFSIGSVVDLYSNIDRELILSKRE ELEGTNGLDENALYHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYLPTVRKCTDGN DFDLRQLRREDITVYVGVNAEDISLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIYAV SEEDDAAKISEKLGYITTTSKSTSKSRGRSTSQGESESEARRALVLPQELGTLDFKEEFI ILKGESPVKAEKALYYLDPYFMDRLMKVSPKLASLTMELNKTKKIFGVKGLKYPSKEKML SVGELESEVLL |
Reference| ID | 71 |
| Plasmid name | pOLA52 |
| GenBank accession number | NC_010378.1 |
| Incompatibility group | IncX1 |
| Genome size | 51602 bp |
| Coordinate of oriT [Strand] | 30771..30796 [+]; 36250..36275 [-] |
| Drug resistance | resistance-nodulation-division (RND)-type efflux-pump (oqxAB); bla encoding beta-lactamase TEM precursor |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Escherichia coli [562] |
Reference| [1] Norman A et al (2008) Nucleotide sequence of pOLA52: a conjugative IncX1 plasmid from Escherichia coli which enables biofilm formation and multidrug efflux. Plasmid. 60(1):59-74. [PMID:18440636] |