oriTDB
The information of the oriT region
oriTDB accession number 100066
Name oriT_pBSDMV46A  insolico
Sequence Completeness core
oriT length 35 nt
oriT sequence CCAGAAGAATTCTGGTATACCGTGTTATACCAAAT
IR (inverted repeat)[1-6] [10-15] nt  (CCAGAA..TTCTGG)
Location of nic site [24-25] nt
Conserved sequence flanking the
  nic site 		TACCGTG|TTAT
Note _
L:1-6;R:10-15;D:24-25
Visualization of oriT structure
Reference
[1] You Y et al (2012) Detection of a common and persistent tet(L)-carrying plasmid in chicken-waste-impacted farm soil. Appl Environ Microbiol. 78(9):3203-13. [PMID:22389375]
I. Information of Relaxase
ID 62
Name Mob_ pBSDMV46A insolico
GenBank accession number YP_006961697
Family MOBV
Length 439 aa
UniProt ID H9B4L1
PDB ID _
Pfam Mob_Pre [PF01076.18], Evalue: 5.30E-37, Aligned region: 1..187
Note nicks plasmid at oriT to initiate conjugation
Protein sequence [Download] MEVYEMAHVQKFTKGNMQGLSIHLDRKTENHSNKNIDTERTHLNYDLCEKDGDTLSRMDE
RLSEVYCMNRKDVKVCCEWIVTLPESLHNESVEYQRKFFEETYEFLTDRYGGQENVVSAN
VHNDETTPHMHFDFVPVVWDEKKEREKVSAKEVLTRSELKSFHTDLDRHLKERIPEIYQE
GILNDKTIGIENIHSLKKYSAEIEQQKEEMANEFKSFKYPQKVLKSIEREVEKKTVGVFN
KKEVVMLPAERYEKMKELSNSSIRIQQRFDKYKSKAVEEIKNLKVNVKNFEEDNEHLRYR
NIDFGREITLLQKERDRQTENAIVYKSILEEKEPDLQISTLEFQGRLVLHNLENDRMPKN
KEEGENWLEILEENKEEKTIPQNRLEKAIGKIKLFLEKFIKRAKEADFSMDWLVEKNKEL
SQQRQQQKKTKSRSSGMEL
Reference
[1] You Y et al (2012) Detection of a common and persistent tet(L)-carrying plasmid in chicken-waste-impacted farm soil. Appl Environ Microbiol. 78(9):3203-13. [PMID:22389375]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 93 _ insolico
 N/A
Information of plasmid
ID 62
Plasmid name pBSDMV46A
GenBank accession number NC_019270.1
Genome size 5031 bp
Coordinate of oriT  [Strand]
Drug resistance insolico resistance to tetracycline
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Bhargavaea cecembensis DMV46A [394098]
Reference
[1] You Y et al (2012) Detection of a common and persistent tet(L)-carrying plasmid in chicken-waste-impacted farm soil. Appl Environ Microbiol. 78(9):3203-13. [PMID:22389375]

Microbial Bioinformatics Group in MML, SJTU