oriTDB
The information of the oriT region
oriTDB accession number 100053
Name oriT_pKLH80  insolico
Sequence Completeness intact
oriT length 117 nt
oriT sequence TAAGCCAGCAGTCACAAGAGGGGGCTATTTTATGCTACGCTAAAAACATCCTATCCCTCT TGTGTGCTGGTTGGGGAGACCCCAAACCCCCTTCAATCTTTTAAGGTGCGTCTTATT
IR (inverted repeat)IR: [28-32] [41-45] nt  (TTTTA..TAAAA)
[71-76] [80-85] nt  (TTGGGG..CCCCAA)
[102-105] [112-115] nt  (TAAG..CTTA)
Location of nic site _
Conserved sequence flanking the
  nic site 		_
Note _
L: 28-32;R:41-45;L:71-76;R:80-85;L:102-105;R:112-115
Visualization of oriT structure
Reference
[1] Petrova M et al (2014) Genetic structure and biological properties of the first ancient multiresistance plasmid pKLH80 isolated from a permafrost bacterium. Microbiology. 160(Pt 10):2253-63. [PMID:25063046]
I. Information of Relaxase
ID 51
Name MobA_pKLH80 insolico
GenBank accession number CCW43458
Family MOBP
Length 388 aa
UniProt ID N1NUY4
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 2.80E-08, Aligned region: 9..289
Note relaxase/mobilization nuclease domain protein,MobA
Protein sequence [Download] MIVKFSKHGKGKASGVLNYLLKEKGSDGVLVPRKHTKVLYGDPVLTEHLIDTAVHKSKYK
SGYLSFSERADEISEDDKKRIMQEFEKVIFCGLEPDQYDILWVEHADKDIDDNNPVGRLE
LNFVIPCQELRTGKSFQPYYEPADQRRVNAWKNIINAEVKTINAERLSDPNDPKRQRLVN
PYNSHAPRPSPFNINSYSKKQSDEDDEIIQHPKSRRDLEEALKRRLLLESQRGVVINRQS
LLTTLRRWGLNVNRSNSETSVSVKHDDLKDKNGRVMSVRLTGGMFAKNYRGIEFKPYIQE
VNSNNYYSEPNKTHRSQLDKMNLEEGIKIKAQYHQDRYKEATLPEPLAIQKAKVTNIVEE
AVDPKGAEQLKYNTPGKLDQKIVANKIE
Reference
[1] Petrova M et al (2014) Genetic structure and biological properties of the first ancient multiresistance plasmid pKLH80 isolated from a permafrost bacterium. Microbiology. 160(Pt 10):2253-63. [PMID:25063046]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 82 MobC_pKLH80 insolico CCW43459 121 aa _
 N/A
Information of plasmid
ID 51
Plasmid name pKLH80
GenBank accession number HF953351.1
Incompatibility group IncP1
Genome size 14835 bp
Coordinate of oriT  [Strand] 1..117 [-]
Drug resistance insolico genes encoding resistance to streptomycin and tetracycline; carbenicillin-hydrolysing beta-lactamase gene: blaRTG-6
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Psychrobacter maritimus MR29-12 [256325]
Reference
[1] Petrova M et al (2014) Genetic structure and biological properties of the first ancient multiresistance plasmid pKLH80 isolated from a permafrost bacterium. Microbiology. 160(Pt 10):2253-63. [PMID:25063046]

Microbial Bioinformatics Group in MML, SJTU