 oriTDB accession number | 100035 |
| Name | oriT_pAoF64/95  |
Sequence Completeness  | core |
| oriT length | 39 nt |
| oriT sequence | CCAAGGGCGCAATTATACGTCGCTGGCGCGACGTGTTGC |
| IR (inverted repeat) | [17-23] [28-34] nt (ACGTCGC..GCGACGT) |
| Location of nic site | [7-8] nt |
| Conserved sequence flanking the nic site | _ |
| Note | similar to oriT regions of pTiC58 |
Visualization of oriT structure Reference| [1] Wetzel ME et al (2014) Quorum-dependent mannopine-inducible conjugative transfer of an Agrobacterium opine-catabolic plasmid. J Bacteriol. 196(5):1031-44. [PMID:24363349] |
| [2] Wetzel ME et al (2015) The repABC Plasmids with Quorum-Regulated Transfer Systems in Members of the Rhizobiales Divide into Two Structurally and Separately Evolving Groups. Genome Biol Evol. 7(12):3337-57. [PMID:26590210] |
| ID | 34 |
| Name | TraA_pAoF64/95 |
| GenBank accession number | AFX65723 |
| Family | MOBQ |
| Length | 1108 aa |
| UniProt ID | K7X7S1 |
| PDB ID | _ |
| Pfam | MobA_MobL [PF03389.14], Evalue: 4.40E-76, Aligned region: 17..243 AAA_30 [PF13604.5], Evalue: 8.70E-57, Aligned region: 386..573 AAA_19 [PF13245.5], Evalue: 3.40E-10, Aligned region: 391..518 Viral_helicase1 [PF01443.17], Evalue: 0.0001, Aligned region: 662..742 |
| Note | be responsible for nicking the oriT |
| Protein sequence [Download] | MAVPHFSVSILARGSGRSAVLSAAYRHCAKMDYEREARTIDYTRKQGLLHEEFVIPADAP DWVRSMIADRSVAGASETFWNKVEGFEKRSDAQLAKDVTIALPLELTAEQNIALMRDFVE RHITAQGMVADWVYHDVPGNPHVHLMTTLRPLTEDGFGSKKVAVLGPDGKPIRNDAGKIV YQLWAGSTEDFNAFRDGWFACQNKHLALAGLDIRIDGRSFEKQGIDLEPTIHLGVGAKAI ERKAEQSDHKSETSTPKLERIELQEERRSENARRIQRRPEIVLDLITREKSVFDERDVAK VLYRYIDDVALFQSLMVRILQSPEALRLERERINFATGIRTPAKYTTREMIRLEAEMANR AIWLSGRASHGVREAVLQATFARHSRLSDEQRTAIEHVAGAPRIAAVIGRAGAGKTTMMK AAREAWETAGYRVVGGALAGKAAEGLEKEAGIVSRTLSSWELRWNQGRNQLDDKTVFVLD EAGMVSSWQMALFVEAVTKVRAKLVLVGDPEQLQPIEAGAAFRAIADRIGYAELETIYRQ RQQWMRDASLDLARGNVRKAVDAYTAHGRMIGLRLKDEAVGSLIAAWDRDYDPSKISLIL AHLRRDVRMLNDMARAKLVERGVVADGFAFKTEDGTRMFAPGDQIVFLKNEGSLGVKNGM LAKVLEAAPGRIVAEVGEGERRQQVTVEQRFYNNLDHGYATTIHKSQGATVDQVKVLASL SLDRHLTYVAMTRHREDLAVYYGTRSFAKSGGLIAILSRRNAKETTLDYEKSALYRQALR FAEARGLHLMNVARTIAHDRLQWAVRQSSRLADLGARLAAIGAALGLVRGSNKHSIPDTI KEAKPMVSGITTFPKSLDQAVEDKLAADPGLKKQWEDVSTRFQLVYAKPEAAFKAIDVDT MLKDQSVAQSTLARIAGEPESFGALKGKTGLLASRGDKQDREKAIANAPALARNLERYLR ERAEAEFKHETEERAVRLKVSVDIPALSPSAKQTLERVRDAIDRNDLPAGLEYALADKMV KAELEGFAKAVSERFGERTFLPLAAKDTDGKTFETATAGMTAGQKAEVQSAWNSMRTVQQ LAAHERTTEALKQAETLRQTKSQGLSLK |
Reference| # | ID | Name | GenBank | Length | Note |
| 1 | 51 | TraC_pAoF64/95 | AFX65722 | 98 aa | Conjugative transfer protein TraC |
| 2 | 52 | TraD_pAoF64/95 | AFX65721 | 71 aa | Conjugative transfer protein TraD |
| ID | 34 |
| Name | TraG_pAoF64/95 |
| SecReT4 accesion number | _ |
| GenBank accession number | AFX65720 |
| Family | VirD4/TraG |
| Length | 644 |
| UniProt ID | K7WNC6 |
| PDB ID | _ |
| Pfam | T4SS-DNA_transf [PF02534.13], Evalue: 1.10E-158, Aligned region: 163..638 TraG-D_C [PF12696.6], Evalue: 3.30E-34, Aligned region: 477..599 TrwB_AAD_bind [PF10412.8], Evalue: 7.60E-20, Aligned region: 225..613 |
| Note | Conjugative transfer coupling protein TraG |
| Protein sequence [Download] | MTINRIALVVVPGTLMILVVVGMTGIEQWLSGFGKTEAARLAWGRVGIALPYGASAAIGI LFLFSSAGSINIRQAGWGVVAGSSGTISIAALRETVRLSAFMKAMPTDNTVLAYLDPATA IGAGVLLMCACFALRVVLIGNAAFARAEPKRIQGKRALHGEADWMKLTEAAKLFPDTGGI VIGEHYRVDKDSVASQAFRSDNAETWGAGGKSPLLCFDGSFGSSHGIVFAGSGGFKTTSV TIPTALKWGSTLIVLDPSNEVAPMVSAHRGGAGRDVFVLDPKKPDIGFNVLDWVGRFGGT KEEDIASVASWIMSDSGGARGVRDDFFRASALQLLTALIADVCLSGHTDETEQTLRQVRM NLSEPEPTLRKRLQDIYDNSGSDFVKENVAAFVNMTPETFSGVYANAVKETHWLSYPNYA ALVSGKKFSTSDIAAGNTDIFINIDLKTLETHSGLARVIIGSFLNAIYNRDGQMKGRALF LLDEVARLGYMRILETARDAGRKYGITLTMIYQSIGQMRETYGGRDAASKWFESASWISF AAINDPETADYISKRCGMTTVEIDQVSRSFQAKGSSRTRSKQLAARPLIQPHEVLRLRAD EQIVFTAGNAPLRCGRAIWFRRDDMKACVGTNRFHMIGDAPKPA |
Reference| ID | 34 |
| Plasmid name | pAoF64/95 |
| GenBank accession number | JX683454.1 |
| Genome size | 176574 bp |
| Coordinate of oriT [Strand] | 156581..156619 [+] |
| Drug resistance | _ |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Agrobacterium tumefaciens F64/95 [358] |
Reference