oriTDB
The information of the oriT region
oriTDB accession number 100021
Name oriT_pDN1  insolico
Sequence Completeness core
oriT length 89 nt
oriT sequence GGCAGTTTCTTGAAGAGAAACCGGTAAGTGCGCCCTCCCGCAAAATGACGGTCGGGGTTT GCGGTGTCTGTGCTCCGATGATAGCATAA
IR (inverted repeat)[5-10] [16-21] nt  (GTTTCT..AGAAAC)
Location of nic site [32-33] nt
Conserved sequence flanking the
  nic site
TAAGTGCG|CCCT
Note _
L:5-10;R:16-21;D:32-33
Visualization of oriT structure
Reference
[1] Whittle G et al (2000) Identification and characterization of a native Dichelobacter nodosus plasmid, pDN1. Plasmid. 43(3):230-4. [PMID:10783302]
[2] Francia MV et al (2004) A classification scheme for mobilization regions of bacterial plasmids. FEMS Microbiol Rev. 28(1):79-100. [PMID:14975531]
I. Information of Relaxase
ID 20
Name MobA_pDN1 insolico
GenBank accession number NP_073212
Family MOBQ
Length 703 aa
UniProt ID Q9EXP9
PDB ID _
Pfam RepB_primase [PF16793.4], Evalue: 7.40E-48, Aligned region: 394..641
MobA_MobL [PF03389.14], Evalue: 4.10E-34, Aligned region: 16..218
Note relaxase
Protein sequence [Download] MAIYHLTAKTGSRNGGQSAKAKADYIQREGRYSRDREEVLHTQSGHLPEWAERPADYWDG
ADLYERANGRLFKEIEFALPVELTLDQQRELVDEFARHLTEGERLPYTLAIHAGNGENPH
CHLMISERKNDGIERPADQWFKRYNGKQPERGGAQKSESLKPKAWLEQTREAWSHYANRA
LEQAGHEARIDHRTLEAQGIERLPGIHLGPNVVEMESRGIRTERADIALAIDTANGQIID
LQEYREVIEHERDRQSEEIQRDQRVSGRDRAAGPEHGDTGGRSPAADRRGTAGQRGAGRE
LDEPATASHERMAAGSKGHEGSRRGTRQGRPDGPERRPWLDMEVVGRGLDRFRDAYSGAA
DRIMALAGAANHRATGEHMADFQAQVKGDRTAQAIARQLKAMGCDRYDIGIRDAASGKMM
NREWTPQEVQQNAAWLKRMNAQGNDIYIRPAEQARHGLVLVDDLSSDDLDAMKQEGREPA
AIIETSPKNYQAWVKVAQDAPADHRGVIARKLAREYDADPASADSRHYGRLAGFTNRKDK
YTSRTGYQPWVLCRESSGKSATAGPELMQQAGQVLDSIERRQERTARLAEITAPQSVRRY
RRSVVDDYRSEMAGLVKRYGDDLSKCDFIAAMKLASKGREADEIGKAMAEASPAIMERKA
GHEADYIKRTVQKVMELPQVQEARAELAKQTQKQRSKGPDLSM
Reference
[1] Whittle G et al (2000) Identification and characterization of a native Dichelobacter nodosus plasmid, pDN1. Plasmid. 43(3):230-4. [PMID:10783302]
[2] Francia MV et al (2004) A classification scheme for mobilization regions of bacterial plasmids. FEMS Microbiol Rev. 28(1):79-100. [PMID:14975531]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 31 MobB_pDN1 insolico NP_073213 136 aa _
2 32 MobC_pDN1 insolico NP_073211 99 aa _
 N/A
Information of plasmid
ID 20
Plasmid name pDN1
GenBank accession number NC_002636.1
Incompatibility group IncQ1
Genome size 5112 bp
Coordinate of oriT  [Strand] 804..892 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Dichelobacter nodosus 1311 [870]
Reference
 N/A