oriTDB
The information of the oriT region
oriTDB accession number 100016
Name oriT_pB10  insolico
Sequence Completeness intact
oriT length 303 nt
oriT sequence TGCCTCGCAGAGCAGGATGACCCGTTGAGCGCCCCCGGCGCGAATAAGGGACAGTGAAGA TAGATAACCGGCTCGCCGGTTAGCTAACTTCACACATCCTGCCCGCCTTACGGCGTTAAT AACACCAAGGAAAGTCTACACCAGCCATTACGATTTATCCGCAACTATCGCGCTATCAGG CCGCAAAAGCAGCAACGGATATAGCGAAAACCGCCACAATGGCCCATAATGCCGCTATCG AAGCGTGCCAATGCACGCCGATAGCGGACTTTTTGCGTTTCCGTAGCGCCGCTTAGTAGC GTT
IR (inverted repeat)[55-72] [75-92] nt  (TGAAGATAGATAACCGGC..GCCGGTTAGCTAACTTCA)
Location of nic site [101-102] nt
Conserved sequence flanking the
  nic site 		CATCCTG|C
Note _
L:55-72;R:75-92;D:101-102
Visualization of oriT structure
Reference
[1] Schlüter A et al (2003) The 64 508 bp IncP-1beta antibiotic multiresistance plasmid pB10 isolated from a waste-water treatment plant provides evidence for recombination between members of different branches of the IncP-1beta group. Microbiology. 149(Pt 11):3139-53. [PMID:14600226]
I. Information of Relaxase
ID 15
Name TraI_pB10 insolico
GenBank accession number CAD97516
Family MOBP
Length 746 aa
UniProt ID Q7X3D1
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 9.90E-64, Aligned region: 12..252
Note DNA relaxase
Protein sequence [Download] MIAKHVPMRSLGKSDFAGLANYITDAQSKDHRLGHVQATNCEAGSIQDAITEVLATQHTN
TRAKGDKTYHLIVSFRAGEQPSADTLRAIEERICVGLGYGEHQRISAVHNDTDNLHIHIA
INKIHPTRHTMHEPYYPHRALAELCTALERDYGLERDNHEPRKRGAEGRAADMERHAGVE
SLVGWIKRECLDEIKGAQSWQELHQVMRDNGMELRVRANGLVFEAGDGTMVKASTVARDL
SKPSLEARLGPFEASPERQAQTTAKRQYRKDPIRLRVNTVELYAKYKAEQQSLTTARAQA
LERARHRKDRLIEAAKRSNRLRRATIKVVGEGRANKKLLYAQASKALRSELQAINKQFQQ
ERTALYAEHSRRTWADWLKKEAQHGGADALAALRAREAAQGLKGNTIRGEGQAKPGHAPA
VDNITKKGTIIFRAGMSAVRDDGDRLQVSREATREGLQEALRLAMQRYGNRITVNGTVEF
KAQMIRAAVDSQLPITFTDPALESRRQALLNKENTHERTERPEHRGRTGRGAGGPGQRPA
ADQHATGAAAVARAGDGRPAAGRGDRADAGLHAATVHRKPDVGRLGRKPPPQSQHRLRAL
SELGVVRIAGGSEVLLPRDVPRHVEQQGTQPDHALRRGISRPGTGVGQTPPGVAAADKYI
AEREAKRLKGFDIPKHSRYTAGDGALTFQGTRTIEGQALALLKRGDEVMVMPIDQATARR
LTRIAVGDAVSITAKGSIKTSKGRSR
Reference
[1] Schlüter A et al (2003) The 64 508 bp IncP-1beta antibiotic multiresistance plasmid pB10 isolated from a waste-water treatment plant provides evidence for recombination between members of different branches of the IncP-1beta group. Microbiology. 149(Pt 11):3139-53. [PMID:14600226]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 23 TraH_pB10 insolico CAD97515 130 aa relaxosome stabilisation protein
2 24 TraJ_pB10 insolico CAD97517 124 aa involved in conjugative DNA transfer
3 25 TraK_pB10 insolico CAD97518 132 aa involved in conjugative DNA transfer
Information of T4CP
ID 15
Name TraG_pB10 insolico
SecReT4 accesion number 31795145
GenBank accession number CAD97514
Family VirD4/TraG
Length 637
UniProt ID Q7X3D2
PDB ID _
Pfam T4SS-DNA_transf [PF02534.13], Evalue: 2.70E-162, Aligned region: 121..624
TraG-D_C [PF12696.6], Evalue: 5.50E-32, Aligned region: 444..569
TrwB_AAD_bind [PF10412.8], Evalue: 5.10E-22, Aligned region: 187..586
Note Type IV Secretion System Coupling Protein
Protein sequence [Download] MKIKMNNAVGPQVRTAKPKPSKLLPVLGAASMVGGLQAATQFFAHTFAYHATLGPNVGHV
YAPWSILHWTYKWYSQYPDEIMKAGSMGMLVSTVGLLGVAVAKVVTSNSSKANEYLHGSA
RWAEKKDIQAAGLLPRERNVLEIVTGKAAPTATGVYVGGWQDKDGNFFYLRHSGPEHVLT
YAPTRSGKGVGLVVPTLLSWGASSVITDLKGELWALTAGWRQKHAKNKVLRFEPASTSGG
VCWNPLDEIRLGTEYEVGDVQNLATLIVDPDGKGLDSHWQKTAFALLVGVILHALYKAKD
DGGTATLPSVDAMLADPNRDIGELWMEMATYGHVDGQNHHAIGSAARDMMDRPEEEAGSV
LSTAKSYLALYRDPVVARNVSRSDFRIKQLMHEDDPVSLYIVTQPNDKARLRPLVRVMVN
MIVRLLADKMDFEGGRPVAHYKHRLLMMLDEFPSLGKLEIMQESLAFVAGYGIKCYLICQ
DINQLKSRETGYGHDESITSNCHVQNAYPPNRVETAEHLSRLTGQTTVVKEQITTSGRRT
AAMLGQVSRTYQEVQRPLLTPDECLRMPGPKKNAQGEIEEAGDMVIYVAGYPAIYGKQPL
YFKDPVFSARAAIPAPKVSDRLRAVAQAETEGEGITI

Reference
[1] Schlüter A et al (2003) The 64 508 bp IncP-1beta antibiotic multiresistance plasmid pB10 isolated from a waste-water treatment plant provides evidence for recombination between members of different branches of the IncP-1beta group. Microbiology. 149(Pt 11):3139-53. [PMID:14600226]
Information of plasmid
ID 15
Plasmid name pB10
GenBank accession number AJ564903.1
Incompatibility group IncP1
Genome size 64508 bp
Coordinate of oriT  [Strand] 31293..31595 [+]
Drug resistance experimental resistance to amoxicillin, streptomycin, sulfonamides and tetracycline
Heavy-metal resistance experimental mercury resistance
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Uncultured bacterium [77133]
Reference
[1] Schlüter A et al (2003) The 64 508 bp IncP-1beta antibiotic multiresistance plasmid pB10 isolated from a waste-water treatment plant provides evidence for recombination between members of different branches of the IncP-1beta group. Microbiology. 149(Pt 11):3139-53. [PMID:14600226]

Microbial Bioinformatics Group in MML, SJTU