 oriTDB accession number | 100009 |
| Name | oriT_R1162  |
Sequence Completeness  | intact |
| oriT length | 160 nt |
| oriT sequence | GGCCAGTTTCTCGAAGAGAAACCGGTAAATGCGCCCTCCCCTACAAAGTAGGGTCGGGAT TGCCGCCGCTGTGCCTCCATGATAGCCTACGAGACAGCACATTAACAATGGGGTGTCAAG ATGGTTAAGGGGAGCAACAAGGCGGCGGATCGGCTGGCCA |
| IR (inverted repeat) | IR1: [3-12] [16-25] nt (CCAGTTTCTC..GAGAAACCGG) IR2: [39-44] [48-53] nt (CCCTAC..GTAGGG) |
| Location of nic site | [33-34] nt |
| Conserved sequence flanking the nic site | TAAATGCG|CCCT |
| Note | _ |
Visualization of oriT structure (The oriT was characterized experimentally) Reference| [1] Meyer R (2009) The R1162 mob proteins can promote conjugative transfer from cryptic origins in the bacterial chromosome. J Bacteriol. 191(5):1574-80. [PMID:19074386] |
| [2] de la Cruz F et al (2010) Conjugative DNA metabolism in Gram-negative bacteria. FEMS Microbiol Rev. 34(1):18-40. [PMID:19919603] |
| [3] Brasch MA et al (1986) Genetic organization of plasmid R1162 DNA involved in conjugative mobilization. J Bacteriol. 167(2):703-10. [PMID:3525520] |
| [4] Zhang S et al (1997) The relaxosome protein MobC promotes conjugal plasmid mobilization by extending DNA strand separation to the nick site at the origin of transfer. Mol Microbiol. 25(3):509-16. [PMID:9302013] |
| [5] Jandle S et al (2006) Stringent and relaxed recognition of oriT by related systems for plasmid mobilization: implications for horizontal gene transfer. J Bacteriol. 188(2):499-506. [PMID:16385040] |
| [6] Becker EC et al (2003) Relaxed specificity of the R1162 nickase: a model for evolution of a system for conjugative mobilization of plasmids. J Bacteriol. 185(12):3538-46. [PMID:12775691] |
| [7] Parker C et al (2005) Elements in the co-evolution of relaxases and their origins of transfer. Plasmid . 53(2):113-8. [PMID:15737398] |
| ID | 8 |
| Name | MobA_R1162 |
| GenBank accession number | P07112 |
| Family | MOBQ |
| Length | 709 aa |
| UniProt ID | P07112 |
| PDB ID | 2NS6 |
| Pfam | |
| Note | to recognize the conserved nick region sequence |
| Protein sequence [Download] | MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVLHAESGHMPEFVERPADYWDA ADLYERANGRLFKEVEFALPVELTLDQQKALASEFAQHLTGAERLPYTLAIHAGGGENPH CHLMISERINDGIERPAAQWFKRYNGKTPEKGGAQKTEALKPKAWLEQTREAWADHANRA LERAGHDARIDHRTLEAQGIERLPGVHLGPNVVEMEGRGIRTDRADVALNIDTANAQIID LQEYREAIDHERNRQSEEIQRHQRVSGADRTAGPEHGDTGRRSPAGHEPDPAGQRGAGGG VAESPAPDRGGMGGAGQRVAGGSRRGEQRRAERPERVAGVALEAMANRDAGFHDAYGGAA DRIVALARPDATDNRGRLDLAALGGPMKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQM MNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREP ALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKD KHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLS RHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAER KPGHEADYIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM |
Reference| [1] Meyer R (2009) The R1162 mob proteins can promote conjugative transfer from cryptic origins in the bacterial chromosome. J Bacteriol. 191(5):1574-80. [PMID:19074386] |
| [2] de la Cruz F et al (2010) Conjugative DNA metabolism in Gram-negative bacteria. FEMS Microbiol Rev. 34(1):18-40. [PMID:19919603] |
| [3] Brasch MA et al (1986) Genetic organization of plasmid R1162 DNA involved in conjugative mobilization. J Bacteriol. 167(2):703-10. [PMID:3525520] |
| [4] Parker C et al (2007) The R1162 relaxase/primase contains two, type IV transport signals that require the small plasmid protein MobB. Mol Microbiol. 66(1):252-61. [PMID:17880426] |
| [5] Zhang S et al (1997) The relaxosome protein MobC promotes conjugal plasmid mobilization by extending DNA strand separation to the nick site at the origin of transfer. Mol Microbiol. 25(3):509-16. [PMID:9302013] |
| [6] Jandle S et al (2006) Stringent and relaxed recognition of oriT by related systems for plasmid mobilization: implications for horizontal gene transfer. J Bacteriol. 188(2):499-506. [PMID:16385040] |
| # | ID | Name | GenBank | Length | Note |
| 1 | 14 | MobB_R1162 | _ | _ | MobB_R1162 stabilizes the relaxase at oriT. R1162 encodes two small proteins required for efficient transfer: MobB (MW 15,115) and MobC (MW 10,885). |
| 2 | 15 | MobC_R1162 | _ | _ | R1162 encodes two small proteins required for efficient transfer: MobB (MW 15,115) and MobC (MW 10,885). |
| ID | 8 |
| Plasmid name | R1162 |
| GenBank accession number | M13380.1 |
| Incompatibility group | IncQ1 |
| Genome size | 8.9 kb |
| Coordinate of oriT [Strand] | 111..270 [+] |
| Drug resistance | resistance to sulfonamides and streptomycin |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Pseudomonas aeruginosa [287] |
Reference| [1] Barth PT et al (1974) Comparison of the deoxyribonucleic acid molecular weights and homologies of plasmids conferring linked resistance to streptomycin and sulfonamides. J Bacteriol. 120(2):618-30. [PMID:4616941] |