T4SS ID	615
Strain	Enterococcus faecalis plasmid pCF10 (NC_006827)
Replicon	plasmid pCF10 [Browse all T4SS(s) in this replicon]
Accession	NC_006827
Location	10015..35356
Name	Prg/Pcf
Function	conjugation
Classification	Type IVD; Type GP
Experimental investigation has been performed on this T4SS.

Information of structure: PcfF, PrgB, PrgB bound to DNA

T4SS components

Component	PcfC	PcfH	PrgA	PrgB	PrgC	PrgD	PrgF	PrgH	PrgI
Number	1	1	1	1	1	1	1	1	1
Component	PrgJ	PrgK	PrgL
Number	1	1	1

The information of T4SS components from NC_006827
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Component
1	pCF10_09 (prgY)	5843..6997 [+], 1155	58616131	PrgY
2	pCF10_10 (prgX)	7030..7983 [-], 954	58616133	PrgX
3	pCF10_11 (prgQ)	8192..8263 [+], 72	58616132	PrgQ
4	pCF10_12 (prgR)	8745..9125 [+], 381	113706805	PrgR
5	pCF10_13 (prgS)	9125..9397 [+], 273	58616086	PrgS
6	pCF10_14 (prgT)	9699..9887 [+], 189	58616137	PrgT
7	pCF10_15 (prgA)	10015..12690 [+], 2676	58616134	PrgA	PrgA
8	pCF10_16 (prgB)	12883..16800 [+], 3918	58616135	PrgB	PrgB
9	pCF10_17 (prgU)	16862..17218 [+], 357	58616087	PrgU
10	pCF10_18 (prgC)	17246..18103 [+], 858	58616136	PrgC	PrgC
11	pCF10_19 (prgD)	18145..19044 [+], 900	58616088	PrgD	PrgD
12	pCF10_20 (prgE)	19064..19498 [+], 435	58616089	PrgE
13	pCF10_21 (prgF)	19545..19772 [+], 228	58616090	PrgF	PrgF
14	pCF10_22 (prgG)	19786..20082 [+], 297	58616091	PrgG
15	pCF10_23 (prgH)	20097..20897 [+], 801	58616084	PrgH	PrgH
16	pCF10_24 (prgI)	20899..21252 [+], 354	58616092	PrgI	PrgI
17	pCF10_25 (prgJ)	21359..23596 [+], 2238	58616093	PrgJ	PrgJ
18	pCF10_26 (prgK)	23608..26223 [+], 2616	58616094	PrgK	PrgK
19	pCF10_27 (prgL)	26247..26873 [+], 627	58616095	PrgL	PrgL
20	pCF10_28 (prgM)	26860..27105 [+], 246	58616096	PrgM
21	pCF10_29 (pcfA)	27089..27697 [+], 609	58616097	PcfA
22	pCF10_30 (pcfB)	27856..28341 [+], 486	113706806	PcfB
23	pCF10_31 (pcfC)	28341..30170 [+], 1830	58616099	PcfC	PcfC
24	pCF10_32 (pcfD)	30221..32380 [+], 2160	58616100	PcfD
25	pCF10_33 (pcfE)	32413..32685 [+], 273	58616101	PcfE
26	pCF10_34 (pcfF)	32931..33287 [+], 357	58616102	PcfF
27	pCF10_35 (pcfG)	33288..34973 [+], 1686	58616103	PcfG
28	pCF10_36 (pcfH)	35006..35356 [+], 351	58616104	PcfH	PcfH
29	pCF10_37 (pcfI)	35680..35847 [+], 168	58616105	PcfI
30	pCF10_38 (pcfJ)	35873..37210 [-], 1338	58616106	PcfJ
31	pCF10_39 (pcfK)	37207..37983 [-], 777	58616107	PcfK
32	pCF10_40 (pcfL)	38126..38674 [-], 549	58616108	PcfL
33	pCF10_41 (pcfM)	38698..38889 [-], 192	58616126	PcfM
34	pCF10_42 (pcfN)	38874..39059 [-], 186	58616109	PcfN
35	pCF10_43 (pcfO)	39065..39514 [-], 450	58616110	PcfO
36	pCF10_44 (pcfP)	39801..40001 [+], 201	58616111	PcfP

 This T4SS contains information of accessory protein.

Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

Download FASTA format files

Proteins        Genes

The information on structure of this T4ss

#	Name	Image	Resource	Detail	Reference
1	PcfF		PDB (6QEQ)	PcfF from Enterococcus faecalis pCF10.	(1) PubMed: 31134011
2	PrgB		PDB (6EVU)	Adhesin domain of PrgB from Enterococcus faecalis.	(2) PubMed: 29723434
3	PrgB bound to DNA		PDB (6GED)	Adhesin domain of PrgB from Enterococcus faecalis bound to DNA.	(3) PubMed: 29723434

(1) Rehman S et al. (2019). Enterococcal PcfF Is a Ribbon-Helix-Helix Protein That Recruits the Relaxase PcfG Through Binding and Bending of the oriT Sequence. Front Microbiol. 10:958. [PudMed:31134011]

(2) Schmitt A et al. (2018). PrgB promotes aggregation, biofilm formation, and conjugation through DNA binding and compaction. Mol Microbiol. 109(3):291-305. [PudMed:29723434]

(3) Schmitt A et al. (2018). PrgB promotes aggregation, biofilm formation, and conjugation through DNA binding and compaction. Mol Microbiol. 109(3):291-305. [PudMed:29723434]

The information on requirements for T4SS substrate-channel docking

Certain T4SS substrates require secretion chaperones for translocation. These chaperones often possess physical properties (small size of 15 kDa, acidic pI, and amphipathic helices) resembling those of chaperones associated with the type III secretion systems, a family of macromolecular translocation systems ancestrally related to bacterial flagella.

#	Accessory protein(GI)	motif(s)	Substrate(s)	Function	Reference
1	PcfF (58616102)	ND (interacts with PcfC T4CP)	PcfG	PcfF interacts with PcfC and PcfG, spatially positions relaxosome or transfer intermediate near the T4CP.	(1) PubMed: 18811729
2	PrgB (58616135)	ND	-	Pheromonedependent overproduction of PrgB induces formation of intercellular aggregates. However, upon overproduction, PrgB confers toxicity on E. faecalis donors by a mechanism dependent on extracellular DNA.	(2) PubMed: 25431047

Tips:
1.Substrate(s): For the conjugation systems, the listed proteins are relaxases that bind a cognate T4CP and are delivered to recipient cells. For the effector translocator systems, the listed proteins are effectors that play a role in the infection processes of the bacterial pathogen.
2.motif(s):The motifs listed are required for substrate translocation. In some cases, the protein or its C-terminal fragment (CT) is sufficient to mediate translocation to target cells, as shown by fusion to a reporter protein such as Cre recombinase or adenylate cyclase. Amino acids (aa) at positions listed relative to the C-terminal fragment (subscript) are required for translocation, as shown by mutational analysis. ND, not determined. Parentheses indicate that the interaction between a protein substrate and a cognate T4CP has been experimentally shown.
3.Accessory protein: Accessory factors required for T4SS channel docking or translocation. The proposed function in mediating substrate-T4SS channel docking is shown in parentheses.PubMed:19946141

(1) Al-Khodor S; Price CT; Habyarimana F; Kalia A; Abu Kwaik Y (2008). A Dot/Icm-translocated ankyrin protein of Legionella pneumophila is required for intracellular proliferation within human macrophages and protozoa. Mol Microbiol. 70(4):908-23. [PudMed:18811729]

(2) Bhatty M et al. (2015). Enterococcus faecalis pCF10-encoded surface proteins PrgA, PrgB (aggregation substance) and PrgC contribute to plasmid transfer, biofilm formation and virulence. Mol Microbiol. 95(4):660-77. [PudMed:25431047]

(1) Li F; Alvarez-Martinez C; Chen Y; Choi KJ; Yeo HJ; Christie PJ (2012). Enterococcus faecalis PrgJ, a VirB4-like ATPase, Mediates pCF10 Conjugative Transfer Through Substrate Binding. J Bacteriol. . [PudMed:22636769]

(2) Chen Y; Zhang X; Manias D; Yeo HJ; Dunny GM; Christie PJ (2008). Enterococcus faecalis PcfC, a spatially localized substrate receptor for type IV secretion of the pCF10 transfer intermediate. J Bacteriol. 190(10):3632-45. [PudMed:18326569]

This literature contains experimental investigation