Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 118267
• Name: oriT_pRBWH1.4
• Organism: Enterococcus faecium strain RBWH1
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP033210 (8483..8521 [-], 39 nt)
• oriT length: 39 nt
• IRs (inverted repeats): 7..15, 18..26 (GCAACATGG..CCATGTTGC)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 39 nt

>oriT_pRBWH1.4
AATATCGCAACATGGGTCCATGTTGCTCTGCTCGCAAAA

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_EBB55_RS16090_pRBWH1.4

• ID: 11610
• GenBank: WP_010782659
• Name: Relaxase_EBB55_RS16090_pRBWH1.4
• UniProt ID: A0JBR7
• Length: 304 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 304 a.a.
• Molecular weight: 35715.46 Da
• Isoelectric Point: 9.8591

>WP_010782659.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Terrabacteria group]
MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRMLYGKDDRVQAHLLIQSFRPGEITAE
KANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHD
LTITERGKKPEKRTMSEIKLKEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFT
YELIHKKKKVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTTEQPE
IRPTRRNHDQHVERGADYQQELSF

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (9-241)

Protein structure

• AlphaFold DB: A0JBR7

Host bacterium

• ID: 18700
• GenBank: NZ_CP033210
• Plasmid name: pRBWH1.4
• Incompatibility group: -
• Plasmid size: 11916 bp
• Coordinate of oriT [Strand]: 8483..8521 [-]
• Host baterium: Enterococcus faecium strain RBWH1

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -