Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   111224
Name   oriT_p1_beb_c250b_NDM5 in_silico
Organism   Enterobacter hormaechei strain beb_c250b
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP095681 (56724..56809 [-], 86 nt)
oriT length   86 nt
IRs (inverted repeats)      61..68, 73..80  (TTGGTGGT..ACCACCAA)
 27..34, 37..44  (GCAAAAAC..GTTTTTGC)
 8..14, 20..26  (TGATTTA..TAAATCA)
Location of nic site      53..54
Conserved sequence flanking the
  nic site    		  TGTGTGGTGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 86 nt

>oriT_p1_beb_c250b_NDM5
AATTACATGATTTAAAACGTAAATCAGCAAAAACTTGTTTTTGCGTAGTGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   7335 GenBank   WP_000948354
Name   traI_KC836_RS00530_p1_beb_c250b_NDM5 insolico UniProt ID   A0AA36L3I8
Length   1756 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1756 a.a.        Molecular weight: 191683.22 Da        Isoelectric Point: 5.6912

>WP_000948354.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacteriaceae]
MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQDPQLHTHVVVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEA
LGYETEVVGKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLQEGMVFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYDRLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVADSPFTALKL
ENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETSLETAISLQKTGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPASERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVVIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEA
QQKAMLKGETFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISKDDGLITLEDAEGNTRLISPREAVAEGVT
LYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVL
EPKPDREVMNAQRLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDSSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGKTEQAVREIAGQERDRADITEREAALPESVLRESQREQEAVREVARENLLQERLQQIERDMVRDLQKE
KTLGGD

  Protein domains


Predicted by InterproScan.

DNA helicase TraI (TraI_C)

(1434-1557)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(633-712)

TrwC relaxase (TrwC)

(10-283)

AAA domain (AAA_30)

(968-1156)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(575-626)


  Protein structure



No available structure.




Auxiliary protein

ID   4068 GenBank   WP_001354030
Name   WP_001354030_p1_beb_c250b_NDM5 insolico UniProt ID   A0A734M5H3
Length   127 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 127 a.a.        Molecular weight: 14508.47 Da        Isoelectric Point: 4.7116

>WP_001354030.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Gammaproteobacteria]
MARVILYISNDVYDKVNAIVEQRRQEGARDKDISVSGTASMLLELGLRVYEAQMERKESAFNQTEFNKLL
LECVVKTQSSVAKILGIESLSPHVSGNPKFEYANMVEDIREKVSSEMERFFPKNDEE

  Protein domains


Predicted by InterproScan.

TraM protein, DNA-binding (Tra_M)

(1-126)


  Protein structure


Source ID Structure
AlphaFold DB A0A734M5H3

ID   4069 GenBank   WP_000089263
Name   WP_000089263_p1_beb_c250b_NDM5 insolico UniProt ID   A0A3U3XJL9
Length   75 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 75 a.a.        Molecular weight: 8541.74 Da        Isoelectric Point: 8.6796

>WP_000089263.1 MULTISPECIES: conjugal transfer relaxosome protein TraY [Gammaproteobacteria]
MSRNIIRPAPGNKVLLVLDDATNHKLLGARERSGRTKTNEVLVRLRDHLNRFPDFYNLDAIKEGAEETDS
IIKDL

  Protein domains


Predicted by InterproScan.

TraY domain (TraY)

(14-60)


  Protein structure


Source ID Structure
AlphaFold DB A0A3U3XJL9


T4CP

ID   8362 GenBank   WP_000069777
Name   traC_KC836_RS00430_p1_beb_c250b_NDM5 insolico UniProt ID   _
Length   876 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 876 a.a.        Molecular weight: 99195.86 Da        Isoelectric Point: 6.1126

>WP_000069777.1 MULTISPECIES: type IV secretion system protein TraC [Enterobacteriaceae]
MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAI
PINGANKSIVEALDHMLRTKLPRGIPLCIHLMSSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAA
ATQFPLPEGMNLPLTLRHYRVFISYCSPSKKKSRADILEMENLVKIIRASLQGASIATQTVDAQAFIDIV
GEMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNPEIAF
LWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWG
ELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGK
GLFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSG
AGKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLS
VMASPNGNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMIVLLDQY
TANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRNLKKLNVIDEGW
RLLDFKNRKVGEFIEKGYRTARRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKY
NQLYPDQFQPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEG
LSIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA

  Protein domains


Predicted by InterproScan.

Family of unknown function (DUF5934) (DUF5934)

(290-447)

TraG P-loop domain (P-loop_TraG)

(468-764)

TraC protein (TraC_F_IV)

(39-277)

  Protein structure



No available structure.



ID   8363 GenBank   WP_046072940
Name   traD_KC836_RS00525_p1_beb_c250b_NDM5 insolico UniProt ID   _
Length   753 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 753 a.a.        Molecular weight: 85667.46 Da        Isoelectric Point: 5.1644

>WP_046072940.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILIGLVLWVKISWQTFINGCIYWWCTSLEGMR
DLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQ
QSENEVTGGRQLTDNPKDVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYAR
QRGDMVVIYDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGDPFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIA
EFAAGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL
KYQARPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDVTQAEQPQQPQQPQQPQQ
PQQPQQPQQPQQPQQPQQPQQPQQPQQPQQPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPP
GISESGEVVDMAAYEAWQQENHPDIQQHMQRREEVNINVHRERGEDVEPGDDF

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(173-560)

F sex factor protein N terminal (TraD_N)

(32-128)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 56156..84298

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KC836_RS00315 (KC836_p000315) 52128..52562 + 435 WP_000845953 conjugation system SOS inhibitor PsiB -
KC836_RS00320 (KC836_p000320) 52559..53278 + 720 WP_001276217 plasmid SOS inhibition protein A -
KC836_RS00325 (KC836_p000325) 53500..53649 + 150 Protein_64 plasmid maintenance protein Mok -
KC836_RS00330 (KC836_p000330) 53591..53716 + 126 WP_001372321 type I toxin-antitoxin system Hok family toxin -
KC836_RS00335 (KC836_p000335) 54035..54331 - 297 Protein_66 hypothetical protein -
KC836_RS00340 (KC836_p000340) 54631..54927 + 297 WP_001272251 hypothetical protein -
KC836_RS00345 (KC836_p000345) 55038..55859 + 822 WP_001234445 DUF932 domain-containing protein -
KC836_RS00350 (KC836_p000350) 56156..56758 - 603 WP_309397939 transglycosylase SLT domain-containing protein virB1
KC836_RS00355 (KC836_p000355) 57081..57464 + 384 WP_001354030 conjugal transfer relaxosome DNA-binding protein TraM -
KC836_RS00360 (KC836_p000360) 57658..58329 + 672 WP_000283561 conjugal transfer transcriptional regulator TraJ -
KC836_RS00365 (KC836_p000365) 58466..58693 + 228 WP_000089263 conjugal transfer relaxosome protein TraY -
KC836_RS00370 (KC836_p000370) 58726..59085 + 360 WP_001098992 type IV conjugative transfer system pilin TraA -
KC836_RS00375 (KC836_p000375) 59100..59411 + 312 WP_000012113 type IV conjugative transfer system protein TraL traL
KC836_RS00380 (KC836_p000380) 59433..59999 + 567 WP_000399780 type IV conjugative transfer system protein TraE traE
KC836_RS00385 (KC836_p000385) 59986..60714 + 729 WP_001230772 type-F conjugative transfer system secretin TraK traK
KC836_RS00390 (KC836_p000390) 60714..62165 + 1452 WP_000146675 F-type conjugal transfer pilus assembly protein TraB traB
KC836_RS00395 (KC836_p000395) 62155..62721 + 567 WP_000896599 conjugal transfer pilus-stabilizing protein TraP -
KC836_RS00400 (KC836_p000400) 62708..63028 + 321 WP_001057307 conjugal transfer protein TrbD -
KC836_RS00405 (KC836_p000405) 63025..63540 + 516 WP_000809881 type IV conjugative transfer system lipoprotein TraV traV
KC836_RS00410 (KC836_p000410) 63675..63896 + 222 WP_001278683 conjugal transfer protein TraR -
KC836_RS00415 (KC836_p000415) 63889..64365 + 477 WP_000549587 hypothetical protein -
KC836_RS00420 (KC836_p000420) 64445..64663 + 219 WP_000556745 hypothetical protein -
KC836_RS00425 (KC836_p000425) 64691..65038 + 348 WP_000836682 hypothetical protein -
KC836_RS00430 (KC836_p000430) 65164..67794 + 2631 WP_000069777 type IV secretion system protein TraC virb4
KC836_RS00435 (KC836_p000435) 67791..68177 + 387 WP_000214084 type-F conjugative transfer system protein TrbI -
KC836_RS00440 (KC836_p000440) 68174..68806 + 633 WP_001203728 type-F conjugative transfer system protein TraW traW
KC836_RS00445 (KC836_p000445) 68803..69795 + 993 WP_000830838 conjugal transfer pilus assembly protein TraU traU
KC836_RS00450 (KC836_p000450) 69825..70130 + 306 WP_000224416 hypothetical protein -
KC836_RS00455 (KC836_p000455) 70139..70777 + 639 WP_001080256 type-F conjugative transfer system pilin assembly protein TrbC trbC
KC836_RS00460 (KC836_p000460) 70774..72624 + 1851 WP_000821856 type-F conjugative transfer system mating-pair stabilization protein TraN traN
KC836_RS00465 (KC836_p000465) 72651..72908 + 258 WP_000864353 conjugal transfer protein TrbE -
KC836_RS00470 (KC836_p000470) 72901..73644 + 744 WP_001030371 type-F conjugative transfer system pilin assembly protein TraF traF
KC836_RS00475 (KC836_p000475) 73658..74002 + 345 WP_000556796 conjugal transfer protein TrbA -
KC836_RS00480 (KC836_p000480) 74121..74405 + 285 WP_000624194 type-F conjugative transfer system pilin chaperone TraQ -
KC836_RS00485 (KC836_p000485) 74392..74936 + 545 Protein_96 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB -
KC836_RS00490 (KC836_p000490) 74866..75213 + 348 WP_001309242 P-type conjugative transfer protein TrbJ -
KC836_RS00495 (KC836_p000495) 75194..75586 + 393 WP_000660699 F-type conjugal transfer protein TrbF -
KC836_RS00500 (KC836_p000500) 75573..76946 + 1374 WP_000944331 conjugal transfer pilus assembly protein TraH traH
KC836_RS00505 (KC836_p000505) 76943..79765 + 2823 WP_001007039 conjugal transfer mating-pair stabilization protein TraG traG
KC836_RS00510 (KC836_p000510) 79781..80266 + 486 WP_000605870 hypothetical protein -
KC836_RS00515 (KC836_p000515) 80315..81046 + 732 WP_000782451 conjugal transfer complement resistance protein TraT -
KC836_RS00520 (KC836_p000520) 81249..81986 + 738 WP_000199905 hypothetical protein -
KC836_RS00525 (KC836_p000525) 82037..84298 + 2262 WP_046072940 type IV conjugative transfer system coupling protein TraD virb4

Host bacterium

ID   11659 GenBank   NZ_CP095681
Plasmid name   p1_beb_c250b_NDM5 Incompatibility group   IncFII
Plasmid size   94643 bp Coordinate of oriT [Strand]   56724..56809 [-]
Host baterium   Enterobacter hormaechei strain beb_c250b

Cargo genes

Drug resistance gene   erm(B), mph(A), blaTEM-1B, rmtB, blaNDM-5, sul1, qacE, aadA2, dfrA12
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIF11