oriTDB
The information of the oriT region
oriTDB accession number 100565
Name oriT_pHS102707  insolico
Sequence Completeness intact
oriT length 103 nt
oriT sequence AGCCTCGCAGAGCAGGATTCCCGTTGAGCAGCCCAGCTGCGAATAAGGGAAGGTAAAGTT GGACCACCGTTTACGGTGGGCCTACTTTACATATCCTGCCCGG
IR (inverted repeat)[53-68] [75-90] nt  (GTAAAGTTGGACCACC..GGTGGGCCTACTT)
Location of nic site [98-99] nt
Conserved sequence flanking the
  nic site 		TATCCTG|C
Note predicted by the oriTfinder
L:53-68;R:75-90;D:98-99
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 637
Name TraI_pHS102707 insolico
GenBank accession number YP_009022955
Family MOBP
Length 869 aa
UniProt ID W6AWK4
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 7.80E-57, Aligned region: 5..245
Note putative relaxase
Protein sequence [Download] MKNIKKSSFSGLADYITNPQSKQERVGVIKATNCQSQDVQDAVTEVNITQRLNTRSESDK
TYHLVISFRPGEKPSEDVLNAIEESICQSLGFGDHQRLSAVHHDTDNLHIHVAINKINPD
TLTIHNPYNDYRVLAESCAYLEKKYGLEVDNHMPTRSASENRAKDMERHSGIQSLLSWVK
AECAEQLLNANSWQELHQAMRQNGLELRQKGNGFVIAGPDGLAVKASSVDRSLSRAALEK
KLGSFESSIPDTKPSRPSPEAIKQRFGSAANVNRPGDRPPPMRENRQVHLGDVPVMQINS
GKRYESKPLGSQGANTTELYGRYQSEQQRLTAARNEALRLAKVKRDRLIENAKRSGRLKR
ATIKLLKGPGVNKRYLYGLASKALGEELERANAIYKKDRQAAYDIHKRRTWADWLQFKAA
GGDDEALKTLRQRNAQQARKEANILHGDKVKKDGRIPGLKPDSVTKEGTIIYRVGESAIR
DGGKRLDVSRDAGDDGLHAALLMAKHRYGDKLTVNGSFEFKKRIVAVAAARELDVSFADP
ELEKLRQILTKKAAREREYIHIPFEKRGKARQAGAMWDTEKKSWFVGPYATRDRIENFIR
MNEEKDNEQRRQRYAGTERTDAGRERSGNGNATGANEPGVTGRSTSADAGNGYGTSSSRR
RTGTGDAVTTAGSQPGRSDDTRTGRSRAAEPNGERGHSKPNVARPGRQPPPTAKNRLRAM
SELGMVRIAGGSEVLLQGNVSDQLEHKTAERADQLRRDMAGAGGITTPAEVKKPLSSAEK
YINEREQKRLKGFDIPKHRGYNSTDKGLFEYAGQRKSGLETLALLKRDDEIVVMPVSDYT
ARRLSNLKVGESVTLTPSGSVRTTKGRSR
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1025
Plasmid name pHS102707
GenBank accession number NC_023907.1
Genome size 69453 bp
Coordinate of oriT  [Strand] 63130..63232 [+]
Drug resistance insolico fosfomycin resistance
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli HS102707 [562]
Reference
[1] Li G et al (2015) First report of a clinical, multidrug-resistant Enterobacteriaceae isolate coharboring fosfomycin resistance gene fosA3 and carbapenemase gene blaKPC-2 on the same transposon, Tn1721. Antimicrob Agents Chemother. 59(1):338-43. [PMID:25367902]

Microbial Bioinformatics Group in MML, SJTU