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the
MS Word file (.doc) or in the
Adobe PDF document (.pdf).
| Introduction Methods Gene Organisms |
| Introduction |
| DNA degradation (which results in a smear pattern) occurs with some bacterial strains during pulsed-field gel electrophoresis (PFGE), like Streptomyces lividans 1326. The DNA degradation (Dnd) phenotype is also frequently determinated by PCR with activated TAE buffer. |
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| Fig. 1. DNA degradation (Dnd) phenotype
under normal agarose gel electrophoresis. Lane 1, 6, 7 and 8 display the
intact DNA after treatment with the activated TAE buffer, whereas Lane 2,
3, 4, 5 and 9 show the Dnd phenotype after treatment. [TOP] |
| Methods |
| Protocol for the Dnd phenotype determination
with activated TAE buffer, in the
MS Word file (.doc) or in the
Adobe PDF document (.pdf). |
| Protocol of pulsed-field gel electrophoresis (PFGE) at protocol-online. |
| A liquid chromatography-coupled tandem quadrupole mass spectrometry approach was descriped in the paper of Wang, et al. (2011). |
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| Analysis of dndABCDE genes responsible for The DNA degradation (Dnd) system in Streptomyces lividans 1326 |
| Mutations in dndA ,dndC, dndD and dndE in wide-type Streptomyces lividans 1326 all abolished the Dnd phenotype while mutation in dndB only aggravates the Dnd phenotype. |
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| Fig. 2. Streptomyces lividans 1326 and its dnd mutants. [Xu et al., BMC Microbiol. 2009 Feb 20;9(1)] |
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| Fig. 3. (A) All of the DNA samples were proven to be stable in normal agarose gel electrophoresis buffer with added thiourea. (B) Genomic DNA from wide-type Streptomyces lividans 1326 and its dndB mutant XTG2 both undergo degradation (streaking in the lane) during gel electrophoresis in Tris-containing buffer. [Xu et al., BMC Microbiol. 2009 Feb 20;9(1)] |
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NCBI_Taxon_ID_230329_277.txt| No. | Organism | Description | Dnd phenotype | Reference |