Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 320414..321027 Replicon chromosome
Accession NZ_CP027721
Organism Pseudomonas chlororaphis subsp. aureofaciens strain ChPhzTR36

Toxin (Protein)


Gene name hicA Uniprot ID A0A285EYZ4
Locus tag C4K12_RS01425 Protein ID WP_009041685.1
Coordinates 320414..320596 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A554PT90
Locus tag C4K12_RS01430 Protein ID WP_009046450.1
Coordinates 320626..321027 (+) Length 134 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C4K12_RS01395 316191..316448 - 258 WP_009046444.1 hypothetical protein -
C4K12_RS01400 316501..316989 - 489 WP_041985368.1 DoxX family protein -
C4K12_RS01405 317063..317958 - 896 Protein_268 SDR family oxidoreductase -
C4K12_RS01410 317987..318496 - 510 WP_009046447.1 DUF1097 domain-containing protein -
C4K12_RS01415 318530..319270 - 741 WP_009046448.1 SDR family oxidoreductase -
C4K12_RS01420 319399..320289 + 891 WP_038630364.1 LysR family transcriptional regulator -
C4K12_RS01425 320414..320596 + 183 WP_009041685.1 type II toxin-antitoxin system HicA family toxin Toxin
C4K12_RS01430 320626..321027 + 402 WP_009046450.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C4K12_RS01435 321596..322633 + 1038 WP_124314665.1 L-glyceraldehyde 3-phosphate reductase -
C4K12_RS01440 322763..323191 - 429 WP_009046452.1 type II toxin-antitoxin system VapC family toxin -
C4K12_RS01445 323188..323433 - 246 WP_009046453.1 plasmid stabilization protein -
C4K12_RS01450 323573..324412 - 840 WP_009046454.1 taurine dioxygenase -
C4K12_RS01455 324648..325487 - 840 WP_009046455.1 taurine ABC transporter permease TauC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6933.08 Da        Isoelectric Point: 10.4588

>T99982 WP_009041685.1 NZ_CP027721:320414-320596 [Pseudomonas chlororaphis subsp. aureofaciens]
MRSRELIRMIEEDGWYLIAVKGSHHQYKHLHKPGRVTIPHPDADLPRGTIHSILKQAGLK

Download         Length: 183 bp

>T99982 NZ_CP027721:320414-320596 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGCGCAGTCGGGAACTGATCAGGATGATCGAGGAGGACGGGTGGTATCTGATCGCGGTCAAAGGCAGTCATCACCAATA
CAAGCATCTGCACAAGCCGGGACGGGTCACTATCCCTCACCCGGATGCGGATCTGCCCAGGGGCACGATCCACAGCATCT
TGAAACAGGCGGGCCTGAAATGA

Antitoxin


Download         Length: 134 a.a.        Molecular weight: 14596.44 Da        Isoelectric Point: 4.9133

>AT99982 WP_009046450.1 NZ_CP027721:320626-321027 [Pseudomonas chlororaphis subsp. aureofaciens]
MKFPVVLHKDADSGYGVIVPDVPGCFSAGHTAAQAFENVKEALSLHYEGLVADGEPLPQVHEVDVHIDNPDYAGGVWGVI
EFDITPYFGKAVRFNATLPEQLLERIDQTVKRDQRYRSRSGFLAAAALRELSA

Download         Length: 402 bp

>AT99982 NZ_CP027721:320626-321027 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGAAATTCCCGGTCGTGCTGCACAAGGATGCCGACTCCGGATATGGAGTGATCGTCCCCGATGTGCCGGGCTGTTTTTC
CGCCGGTCATACCGCTGCGCAAGCCTTCGAGAATGTGAAAGAGGCCCTTTCGCTGCATTATGAAGGCCTGGTCGCCGATG
GTGAGCCGCTGCCCCAGGTGCATGAGGTCGACGTGCATATCGACAATCCTGATTACGCCGGTGGCGTGTGGGGCGTGATC
GAGTTCGACATCACGCCTTACTTCGGCAAGGCGGTGCGTTTCAATGCGACGTTGCCGGAGCAACTGTTGGAGCGCATCGA
CCAGACGGTGAAGCGCGACCAGCGCTACCGTTCGCGCTCGGGCTTTCTCGCGGCAGCGGCATTGCGCGAACTCTCCGCCT
AG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A285EYZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A554PT90

References