Detailed information of TA system TA099925

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 6122030..6122619
• Replicon: chromosome
• Accession: NZ_CP027711
• Organism: Pseudomonas chlororaphis subsp. piscium strain ChPhzTR44

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: C4K28_RS27555
• Protein ID: WP_016702674.1
• Coordinates: 6122320..6122619 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: C4K28_RS27550
• Protein ID: WP_053281098.1
• Coordinates: 6122030..6122323 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K28_RS27520	6117110..6117643	+	534	WP_025807757.1	2,4'-dihydroxyacetophenone dioxygenase family protein	-
C4K28_RS27525	6117818..6118612	+	795	WP_025807760.1	SDR family NAD(P)-dependent oxidoreductase	-
C4K28_RS27530	6118791..6119471	+	681	WP_025807762.1	isochorismatase family protein	-
C4K28_RS27535	6119657..6120061	+	405	WP_063433159.1	DoxX family protein	-
C4K28_RS27540	6120268..6120660	+	393	WP_063433160.1	DUF3077 domain-containing protein	-
C4K28_RS27545	6120861..6122024	+	1164	WP_063433161.1	GGDEF domain-containing protein	-
C4K28_RS27550	6122030..6122323	-	294	WP_053281098.1	putative addiction module antidote protein	Antitoxin
C4K28_RS27555	6122320..6122619	-	300	WP_016702674.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K28_RS27560	6122793..6123242	+	450	WP_063433162.1	DUF3828 domain-containing protein	-
C4K28_RS27565	6123267..6124277	-	1011	WP_196762739.1	NAD(P)-dependent alcohol dehydrogenase	-
C4K28_RS27570	6124333..6124632	-	300	WP_007924100.1	ArsR family transcriptional regulator	-
C4K28_RS27575	6124699..6125787	-	1089	WP_025807778.1	nitrite reductase, copper-containing	-
C4K28_RS27580	6126014..6126256	+	243	WP_007924102.1	exodeoxyribonuclease VII small subunit	-
C4K28_RS27585	6126253..6127140	+	888	WP_053281103.1	(2E,6E)-farnesyl diphosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11221.93 Da
• Isoelectric Point: 10.6086

>T99925 WP_016702674.1 NZ_CP027711:c6122619-6122320 [Pseudomonas chlororaphis subsp. piscium]
MIEIKQTATFMEWENRLKDSRARALIAARIFRLANGLAGDVSPVGEGVSELRIHYGPGYRVYFQQLGSQLVILLCGGDKS
RQSRDIELAKQLARRWSRS

Nucleotide

• Length: 300 bp

>T99925 NZ_CP027711:c6122619-6122320 [Pseudomonas chlororaphis subsp. piscium]
ATGATCGAGATCAAACAGACCGCGACCTTTATGGAGTGGGAAAACAGGCTCAAGGATTCCAGAGCCAGGGCGTTGATCGC
TGCGCGGATTTTTCGCCTGGCCAACGGCCTTGCCGGTGACGTGTCACCGGTGGGCGAGGGCGTCAGTGAGCTGCGCATCC
ATTACGGGCCGGGCTACCGGGTTTACTTCCAGCAGCTTGGTTCGCAACTTGTCATTCTGTTGTGCGGCGGCGACAAGAGT
CGCCAGAGCCGCGATATCGAACTGGCCAAACAGCTGGCCCGTCGTTGGAGCCGATCATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10584.16 Da
• Isoelectric Point: 6.4627

>AT99925 WP_053281098.1 NZ_CP027711:c6122323-6122030 [Pseudomonas chlororaphis subsp. piscium]
MNEPLFDYDPATSLRDPQAIALFMADAFETGDAGYIAKALGVVARAKGMTELAHETGLSREQLYRSFSDKGNPTLKTLLA
VMRALGLDMTARVHVRP

Nucleotide

• Length: 294 bp

>AT99925 NZ_CP027711:c6122323-6122030 [Pseudomonas chlororaphis subsp. piscium]
ATGAACGAACCGCTTTTCGACTATGACCCCGCCACGTCCCTGCGTGACCCGCAGGCCATTGCCCTGTTCATGGCGGACGC
CTTCGAGACCGGCGATGCCGGCTACATCGCCAAAGCCCTGGGCGTGGTGGCGCGGGCCAAGGGCATGACCGAGCTGGCCC
ATGAAACCGGGCTGTCCCGCGAGCAGCTGTATCGCTCGTTCAGCGACAAGGGCAACCCGACCTTGAAGACCCTGCTGGCG
GTGATGCGTGCCCTGGGGCTGGACATGACGGCGCGGGTGCATGTCAGGCCGTAG