Detailed information of TA system TA099920

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 311667..312280
• Replicon: chromosome
• Accession: NZ_CP027711
• Organism: Pseudomonas chlororaphis subsp. piscium strain ChPhzTR44

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A285EYZ4
• Locus tag: C4K28_RS01370
• Protein ID: WP_009041685.1
• Coordinates: 311667..311849 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C4K28_RS01375
• Protein ID: WP_025808441.1
• Coordinates: 311879..312280 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K28_RS01345	306856..308820	+	1965	WP_164486263.1	choline transporter BetT	-
C4K28_RS01350	308936..309193	-	258	WP_038583011.1	hypothetical protein	-
C4K28_RS01355	309246..309725	-	480	WP_124372905.1	DoxX family protein	-
C4K28_RS01360	309782..310522	-	741	WP_063427782.1	SDR family oxidoreductase	-
C4K28_RS01365	310652..311542	+	891	WP_025808442.1	LysR family transcriptional regulator	-
C4K28_RS01370	311667..311849	+	183	WP_009041685.1	type II toxin-antitoxin system HicA family toxin	Toxin
C4K28_RS01375	311879..312280	+	402	WP_025808441.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4K28_RS01380	312869..313906	+	1038	WP_063427783.1	L-glyceraldehyde 3-phosphate reductase	-
C4K28_RS01385	314036..314464	-	429	WP_009046452.1	type II toxin-antitoxin system VapC family toxin	-
C4K28_RS01390	314461..314706	-	246	WP_009046453.1	plasmid stabilization protein	-
C4K28_RS01395	314846..315685	-	840	WP_106696248.1	taurine dioxygenase	-
C4K28_RS01400	315923..316762	-	840	WP_063427785.1	taurine ABC transporter permease TauC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6933.08 Da
• Isoelectric Point: 10.4588

>T99920 WP_009041685.1 NZ_CP027711:311667-311849 [Pseudomonas chlororaphis subsp. piscium]
MRSRELIRMIEEDGWYLIAVKGSHHQYKHLHKPGRVTIPHPDADLPRGTIHSILKQAGLK

Nucleotide

• Length: 183 bp

>T99920 NZ_CP027711:311667-311849 [Pseudomonas chlororaphis subsp. piscium]
ATGCGCAGTCGGGAACTGATCAGGATGATCGAGGAGGACGGGTGGTATCTGATCGCGGTCAAAGGCAGTCATCACCAGTA
CAAACATCTGCACAAGCCGGGGCGGGTCACTATCCCTCACCCGGATGCGGATCTGCCCAGGGGCACGATCCACAGCATCT
TGAAACAGGCGGGCCTGAAATGA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14568.38 Da
• Isoelectric Point: 4.9036

>AT99920 WP_025808441.1 NZ_CP027711:311879-312280 [Pseudomonas chlororaphis subsp. piscium]
MKFPVVLHKDADSGYGVIVPDVPGCFSAGHTAAQAFENVKEALSLHYEGLVADGEPLPQVHEVDVHIDNPDYAGGVWGVV
DFDITPYFGKAVRFNATLPEQLLERIDQTVKRDQRYRSRSGFLAAAALRELSA

Nucleotide

• Length: 402 bp

>AT99920 NZ_CP027711:311879-312280 [Pseudomonas chlororaphis subsp. piscium]
ATGAAATTCCCGGTCGTGCTGCACAAGGATGCCGACTCCGGATATGGAGTGATCGTCCCCGATGTGCCGGGCTGTTTTTC
CGCCGGTCATACCGCTGCGCAAGCCTTCGAGAATGTGAAAGAAGCCTTGTCGCTGCACTACGAAGGCCTGGTCGCCGATG
GTGAGCCGCTGCCCCAGGTACATGAGGTTGATGTGCATATCGACAATCCCGATTACGCCGGCGGTGTATGGGGGGTGGTC
GATTTCGATATCACGCCTTACTTCGGCAAGGCGGTGCGTTTTAATGCGACGTTGCCGGAGCAACTGTTGGAGCGCATCGA
CCAGACAGTGAAGCGCGACCAGCGCTACCGTTCGCGCTCGGGTTTTCTCGCCGCGGCGGCACTACGCGAGCTCTCCGCCT
AG

Structures

Toxin

• AlphaFold DB: A0A285EYZ4