Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3868152..3868693 Replicon chromosome
Accession NZ_CP027591
Organism Escherichia coli strain 2014C-3011

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag C7A05_RS22295 Protein ID WP_000615979.1
Coordinates 3868415..3868693 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag C7A05_RS22290 Protein ID WP_000729704.1
Coordinates 3868152..3868412 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7A05_RS22260 3864398..3864616 - 219 Protein_3911 GNAT family N-acetyltransferase -
C7A05_RS22265 3864675..3865369 + 695 WP_085949012.1 IS1 family transposase -
C7A05_RS22270 3865385..3865624 - 240 Protein_3913 GNAT family N-acetyltransferase -
C7A05_RS22275 3865621..3866676 - 1056 WP_001226164.1 DNA polymerase IV -
C7A05_RS22280 3866747..3867532 - 786 WP_062882563.1 putative lateral flagellar export/assembly protein LafU -
C7A05_RS22285 3867504..3867865 + 362 Protein_3916 FHIPEP family type III secretion protein -
C7A05_RS29850 3867856..3867966 - 111 Protein_3917 peptidoglycan endopeptidase -
C7A05_RS22290 3868152..3868412 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
C7A05_RS22295 3868415..3868693 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
C7A05_RS22300 3868849..3869589 + 741 WP_001225679.1 murein L,D-transpeptidase -
C7A05_RS22305 3869560..3870327 - 768 WP_001365365.1 class II glutamine amidotransferase -
C7A05_RS22310 3870533..3871111 - 579 WP_062882565.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T99700 WP_000615979.1 NZ_CP027591:3868415-3868693 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T99700 NZ_CP027591:3868415-3868693 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT99700 WP_000729704.1 NZ_CP027591:3868152-3868412 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT99700 NZ_CP027591:3868152-3868412 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References