Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 5030340..5031139 Replicon chromosome
Accession NZ_CP027582
Organism Escherichia coli strain 2013C-4538

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag C6999_RS27265 Protein ID WP_000347273.1
Coordinates 5030340..5030804 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag C6999_RS27270 Protein ID WP_001307405.1
Coordinates 5030804..5031139 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6999_RS27230 5025341..5025775 - 435 WP_000948822.1 PTS sugar transporter subunit IIA -
C6999_RS27235 5025793..5026671 - 879 WP_001341890.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
C6999_RS27240 5026661..5027440 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
C6999_RS27245 5027451..5027924 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
C6999_RS27250 5027947..5029227 - 1281 WP_000681914.1 tagatose-bisphosphate aldolase subunit KbaZ -
C6999_RS27260 5029476..5030285 + 810 WP_000072193.1 aga operon transcriptional regulator AgaR -
C6999_RS27265 5030340..5030804 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
C6999_RS27270 5030804..5031139 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
C6999_RS27275 5031288..5032859 - 1572 WP_001273779.1 galactarate dehydratase -
C6999_RS27280 5033234..5034568 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
C6999_RS27285 5034584..5035354 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T99594 WP_000347273.1 NZ_CP027582:c5030804-5030340 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T99594 NZ_CP027582:c5030804-5030340 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT99594 WP_001307405.1 NZ_CP027582:c5031139-5030804 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT99594 NZ_CP027582:c5031139-5030804 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
ACCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References