Detailed information of TA system TA099549

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 2268295..2269132
• Replicon: chromosome
• Accession: NZ_CP027579
• Organism: Escherichia coli strain 2013C-4282

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: V0SED1
• Locus tag: C6996_RS11600
• Protein ID: WP_000227787.1
• Coordinates: 2268590..2269132 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: B1LJI1
• Locus tag: C6996_RS11595
• Protein ID: WP_001353405.1
• Coordinates: 2268295..2268606 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6996_RS11570	2263315..2264262	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
C6996_RS11575	2264284..2266275	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
C6996_RS11580	2266265..2266879	+	615	WP_000179807.1	cytochrome o ubiquinol oxidase subunit III	-
C6996_RS11585	2266879..2267208	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
C6996_RS11590	2267220..2268110	+	891	WP_000971336.1	heme o synthase	-
C6996_RS11595	2268295..2268606	+	312	WP_001353405.1	DUF1778 domain-containing protein	Antitoxin
C6996_RS11600	2268590..2269132	+	543	WP_000227787.1	GNAT family N-acetyltransferase	Toxin
C6996_RS11605	2269188..2270123	-	936	WP_000932788.1	sel1 repeat family protein	-
C6996_RS11610	2270540..2271904	+	1365	WP_001000980.1	MFS transporter	-
C6996_RS11615	2272032..2272523	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
C6996_RS11620	2272691..2273602	+	912	WP_000705835.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-96)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19946.24 Da
• Isoelectric Point: 8.8951

>T99549 WP_000227787.1 NZ_CP027579:2268590-2269132 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
RPNVFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T99549 NZ_CP027579:2268590-2269132 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCTGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAGATTGGTGTTGTCAGATTAGTCATGTTAGGGGTAGCGCG
GAAATATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCGGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATACTCGTCTCGGCTTTGTTCAGCTTTCAGCG
AGGCCCAATGTTTTCGGTGCTGTACCGATGTTTTTAGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11370.09 Da
• Isoelectric Point: 10.4825

>AT99549 WP_001353405.1 NZ_CP027579:2268295-2268606 [Escherichia coli]
MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT99549 NZ_CP027579:2268295-2268606 [Escherichia coli]
ATGCCTGGAAAAACAGTCGCTCTACCCGATATCGATAAAACGCTGAAAAATGCGCGGGTTGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCAGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A829GE43

Antitoxin

• AlphaFold DB: A0A829G7G3