Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4245688..4246190 Replicon chromosome
Accession NZ_CP027577
Organism Escherichia coli strain 2013C-4225

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C6997_RS23335 Protein ID WP_000767829.1
Coordinates 4245688..4245942 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C6997_RS23340 Protein ID WP_001259255.1
Coordinates 4245939..4246190 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6997_RS23305 4240703..4240930 - 228 WP_000985280.1 sulfurtransferase TusA family protein -
C6997_RS23310 4240944..4242002 - 1059 WP_000492346.1 transport protein YeeE -
C6997_RS23315 4242181..4243539 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C6997_RS23320 4243529..4243591 - 63 WP_010723108.1 membrane protein YoeI -
C6997_RS23325 4243806..4244735 - 930 WP_000803351.1 LysR family transcriptional regulator -
C6997_RS23330 4244781..4245605 - 825 WP_000754737.1 SDR family oxidoreductase -
C6997_RS23335 4245688..4245942 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C6997_RS23340 4245939..4246190 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C6997_RS23345 4246473..4246523 + 51 WP_001368802.1 his operon leader peptide -
C6997_RS23355 4246669..4247568 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
C6997_RS23360 4247574..4248878 + 1305 WP_001298841.1 histidinol dehydrogenase -
C6997_RS23365 4248875..4249945 + 1071 WP_000108980.1 histidinol-phosphate transaminase -
C6997_RS23370 4249945..4251012 + 1068 WP_000080085.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T99531 WP_000767829.1 NZ_CP027577:c4245942-4245688 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T99531 NZ_CP027577:c4245942-4245688 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT99531 WP_001259255.1 NZ_CP027577:c4246190-4245939 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT99531 NZ_CP027577:c4246190-4245939 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References