Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 1026193..1026664 Replicon chromosome
Accession NZ_CP027573
Organism Escherichia coli strain 2013C-4081

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag A2F94_RS05700 Protein ID WP_001303511.1
Coordinates 1026386..1026664 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag A2F94_RS05695 Protein ID WP_001302048.1
Coordinates 1026193..1026384 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A2F94_RS05650 1021666..1021938 - 273 WP_012817871.1 hypothetical protein -
A2F94_RS05660 1022106..1022246 - 141 WP_072058819.1 type I toxin-antitoxin system Hok family toxin -
A2F94_RS05665 1022284..1023497 + 1214 WP_162829202.1 IS3 family transposase -
A2F94_RS05670 1023537..1023953 - 417 WP_023981635.1 DUF977 family protein -
A2F94_RS05675 1023994..1024956 - 963 WP_000095671.1 helix-turn-helix domain-containing protein -
A2F94_RS05680 1024979..1025404 - 426 WP_000693943.1 Rha family transcriptional regulator -
A2F94_RS05685 1025401..1025703 - 303 WP_001444689.1 transcriptional regulator -
A2F94_RS05690 1025801..1026172 + 372 WP_001169687.1 hypothetical protein -
A2F94_RS05695 1026193..1026384 + 192 WP_001302048.1 hypothetical protein Antitoxin
A2F94_RS05700 1026386..1026664 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
A2F94_RS05710 1026960..1027349 + 390 WP_001447517.1 hypothetical protein -
A2F94_RS05715 1027390..1027608 + 219 WP_001171930.1 hypothetical protein -
A2F94_RS05725 1028092..1028280 + 189 WP_000449192.1 cell division inhibition protein DicB -
A2F94_RS05730 1028277..1028468 + 192 WP_001090200.1 DUF1482 family protein -
A2F94_RS05735 1028561..1031026 + 2466 WP_000048442.1 exonuclease -
A2F94_RS05740 1031088..1031357 + 270 WP_000003742.1 excisionase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - espK / espN / espX7/nleL 974655..1039613 64958


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T99482 WP_001303511.1 NZ_CP027573:1026386-1026664 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T99482 NZ_CP027573:1026386-1026664 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT99482 WP_001302048.1 NZ_CP027573:1026193-1026384 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT99482 NZ_CP027573:1026193-1026384 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References