Detailed information of TA system TA099458

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4635351..4635976
• Replicon: chromosome
• Accession: NZ_CP027555
• Organism: Escherichia coli strain 2013C-3513

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C6991_RS25470
• Protein ID: WP_000911330.1
• Coordinates: 4635578..4635976 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: C6991_RS25465
• Protein ID: WP_000450524.1
• Coordinates: 4635351..4635578 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6991_RS25440	4631154..4631624	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
C6991_RS25445	4631624..4632196	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
C6991_RS25450	4632342..4633220	+	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
C6991_RS25455	4633237..4634271	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
C6991_RS25460	4634484..4635197	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
C6991_RS25465	4635351..4635578	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
C6991_RS25470	4635578..4635976	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
C6991_RS25475	4636123..4636986	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
C6991_RS25480	4637001..4639016	+	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
C6991_RS25485	4639090..4639788	+	699	WP_000679812.1	esterase	-
C6991_RS25490	4639898..4640098	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T99458 WP_000911330.1 NZ_CP027555:4635578-4635976 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T99458 NZ_CP027555:4635578-4635976 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT99458 WP_000450524.1 NZ_CP027555:4635351-4635578 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT99458 NZ_CP027555:4635351-4635578 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33