Detailed information of TA system TA099321

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3996921..3997523
• Replicon: chromosome
• Accession: NZ_CP027546
• Organism: Escherichia coli strain 2013C-4187

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: C6993_RS21790
• Protein ID: WP_000897305.1
• Coordinates: 3996921..3997232 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6993_RS21795
• Protein ID: WP_000356397.1
• Coordinates: 3997233..3997523 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6993_RS21760	3991951..3992736	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
C6993_RS21765	3992835..3993434	+	600	WP_001315111.1	glucose-1-phosphatase	-
C6993_RS21770	3993428..3994300	+	873	WP_000920762.1	virulence factor BrkB family protein	-
C6993_RS21775	3994297..3994734	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
C6993_RS21780	3994779..3995720	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
C6993_RS21785	3995784..3996692	-	909	WP_001162704.1	alpha/beta hydrolase	-
C6993_RS21790	3996921..3997232	+	312	WP_000897305.1	hypothetical protein	Toxin
C6993_RS21795	3997233..3997523	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
C6993_RS21805	3998128..3998346	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
C6993_RS21810	3998565..3998807	+	243	WP_001086390.1	hypothetical protein	-
C6993_RS21815	3999036..4000016	-	981	WP_000399648.1	IS110-like element IS621 family transposase	-
C6993_RS21825	4000416..4001345	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
C6993_RS21830	4001342..4001977	-	636	WP_000829008.1	formate dehydrogenase cytochrome b556 subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3999036..4000016	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T99321 WP_000897305.1 NZ_CP027546:3996921-3997232 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T99321 NZ_CP027546:3996921-3997232 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT99321 WP_000356397.1 NZ_CP027546:3997233-3997523 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT99321 NZ_CP027546:3997233-3997523 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7