Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1826027..1826529 Replicon chromosome
Accession NZ_CP027546
Organism Escherichia coli strain 2013C-4187

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C6993_RS10530 Protein ID WP_000767829.1
Coordinates 1826027..1826281 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C6993_RS10535 Protein ID WP_001259255.1
Coordinates 1826278..1826529 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6993_RS10500 1821042..1821269 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
C6993_RS10505 1821283..1822341 - 1059 WP_000492323.1 transport protein YeeE -
C6993_RS10510 1822520..1823878 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C6993_RS10515 1823868..1823930 - 63 WP_010723108.1 membrane protein YoeI -
C6993_RS10520 1824145..1825074 - 930 WP_000803341.1 LysR family transcriptional regulator -
C6993_RS10525 1825120..1825944 - 825 WP_000754755.1 SDR family oxidoreductase -
C6993_RS10530 1826027..1826281 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C6993_RS10535 1826278..1826529 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C6993_RS10540 1826813..1826863 + 51 WP_001364200.1 his operon leader peptide -
C6993_RS10550 1827009..1827908 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
C6993_RS10555 1827914..1829218 + 1305 WP_001484680.1 histidinol dehydrogenase -
C6993_RS10560 1829215..1830285 + 1071 WP_001485892.1 histidinol-phosphate transaminase -
C6993_RS10565 1830285..1831352 + 1068 WP_000080097.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T99315 WP_000767829.1 NZ_CP027546:c1826281-1826027 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T99315 NZ_CP027546:c1826281-1826027 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT99315 WP_001259255.1 NZ_CP027546:c1826529-1826278 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT99315 NZ_CP027546:c1826529-1826278 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References