Detailed information of TA system TA099275

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1474100..1474725
• Replicon: chromosome
• Accession: NZ_CP027544
• Organism: Escherichia coli strain 2013C-3264

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MJ68_RS08420
• Protein ID: WP_000911330.1
• Coordinates: 1474100..1474498 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: MJ68_RS08425
• Protein ID: WP_000450524.1
• Coordinates: 1474498..1474725 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ68_RS08400	1469978..1470178	+	201	WP_000383836.1	YpfN family protein	-
MJ68_RS08405	1470288..1470986	-	699	WP_000679823.1	esterase	-
MJ68_RS08410	1471060..1473075	-	2016	WP_000829387.1	tRNA cytosine(34) acetyltransferase TmcA	-
MJ68_RS08415	1473090..1473953	-	864	WP_001267496.1	neutral zinc metallopeptidase	-
MJ68_RS08420	1474100..1474498	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
MJ68_RS08425	1474498..1474725	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
MJ68_RS08430	1474879..1475592	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
MJ68_RS08435	1475805..1476839	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
MJ68_RS08440	1476856..1477734	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
MJ68_RS08445	1477880..1478452	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
MJ68_RS08450	1478452..1478922	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T99275 WP_000911330.1 NZ_CP027544:c1474498-1474100 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T99275 NZ_CP027544:c1474498-1474100 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT99275 WP_000450524.1 NZ_CP027544:c1474725-1474498 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT99275 NZ_CP027544:c1474725-1474498 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33