Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pezAT/zeta-ParD
Location 3630228..3631224 Replicon chromosome
Accession NZ_CP027541
Organism Mycolicibacterium smegmatis MKD8

Toxin (Protein)


Gene name pezT Uniprot ID A0A2U9PRH2
Locus tag D806_RS17480 Protein ID WP_003894880.1
Coordinates 3630646..3631224 (+) Length 193 a.a.

Antitoxin (Protein)


Gene name pezA Uniprot ID I7GB97
Locus tag D806_RS17475 Protein ID WP_003894879.1
Coordinates 3630228..3630620 (+) Length 131 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D806_RS17455 3625699..3626817 + 1119 WP_003894875.1 acyltransferase -
D806_RS17460 3626990..3627595 + 606 WP_003894876.1 class I SAM-dependent methyltransferase -
D806_RS17465 3627597..3629171 - 1575 WP_198670975.1 GMC family oxidoreductase N-terminal domain-containing protein -
D806_RS17470 3629535..3630164 + 630 WP_003894878.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
D806_RS17475 3630228..3630620 + 393 WP_003894879.1 hypothetical protein Antitoxin
D806_RS17480 3630646..3631224 + 579 WP_003894880.1 zeta toxin family protein Toxin
D806_RS17485 3631236..3632588 - 1353 WP_003894881.1 LysM peptidoglycan-binding domain-containing protein -
D806_RS17490 3632923..3633078 + 156 WP_003894882.1 hypothetical protein -
D806_RS17495 3633276..3634664 + 1389 WP_003894883.1 L-serine ammonia-lyase -
D806_RS17500 3634698..3635258 - 561 WP_051065174.1 hypothetical protein -
D806_RS17505 3635418..3636224 + 807 WP_036453221.1 transporter substrate-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(4-136)

Antitoxin

ParD-like antitoxin of type II bacterial toxin-antitoxin system

(6-106)


Sequences

Toxin        


Download         Length: 193 a.a.        Molecular weight: 21119.30 Da        Isoelectric Point: 9.5596

>T99270 WP_003894880.1 NZ_CP027541:3630646-3631224 [Mycolicibacterium smegmatis MKD8]
VKRLDLVVGPNGAGKSTFVELTLAPLLTGSAFVNADEIAKRRWPHAPAEHSYDAARIASDTRLKLIELGESFIAETVFSH
PSKIELIDTAQAAGYTVVMHVVMIPEDLAVLRVQHRVRAGGHHVPEEKIRERHQRLWPLVALAAARADISTFYDNSAVRG
PRIVAQLAGGFAVGRPAWPMWAPQVLTSRWPE

Download         Length: 579 bp

>T99270 NZ_CP027541:3630646-3631224 [Mycolicibacterium smegmatis MKD8]
GTGAAACGACTCGATCTCGTCGTTGGGCCTAACGGCGCGGGCAAGAGCACTTTCGTCGAACTCACATTGGCCCCGCTGCT
GACGGGCAGTGCGTTCGTCAACGCCGACGAGATCGCCAAGCGTCGCTGGCCGCACGCCCCGGCCGAGCACTCCTACGATG
CCGCACGCATCGCCTCCGACACCCGACTCAAGCTGATCGAACTCGGTGAATCGTTCATCGCCGAAACGGTGTTCTCACAT
CCCTCGAAAATTGAGCTCATCGACACCGCGCAGGCGGCTGGATACACCGTGGTAATGCACGTGGTGATGATCCCCGAGGA
TCTCGCCGTCTTACGCGTGCAGCACCGGGTACGAGCCGGTGGTCACCACGTGCCCGAGGAGAAGATCCGCGAACGGCACC
AACGACTCTGGCCCCTCGTCGCGCTGGCGGCCGCCCGCGCCGACATCTCGACGTTCTATGACAACAGCGCCGTCAGGGGT
CCGCGCATCGTTGCCCAACTCGCCGGCGGATTCGCTGTGGGCCGGCCCGCCTGGCCGATGTGGGCACCCCAGGTGCTCAC
CTCGCGGTGGCCCGAATAG

Antitoxin


Download         Length: 131 a.a.        Molecular weight: 13841.29 Da        Isoelectric Point: 4.9246

>AT99270 WP_003894879.1 NZ_CP027541:3630228-3630620 [Mycolicibacterium smegmatis MKD8]
MTETADRVTRFAADLVESAAAEGARQSRSAKQQLDHWARVGRAVSSQHTAARRRVEAALAGDLALRDLTPEEGVVFNAEI
SAAIEENLAHTDYGQVLAGRGVTTVALDDDGAIVRYQPDGTTTVLAPAQR

Download         Length: 393 bp

>AT99270 NZ_CP027541:3630228-3630620 [Mycolicibacterium smegmatis MKD8]
ATGACTGAGACCGCAGATCGGGTCACTCGCTTCGCCGCAGACCTGGTGGAGAGCGCCGCGGCCGAAGGCGCACGTCAGAG
TAGGTCGGCCAAGCAGCAACTCGACCATTGGGCACGAGTGGGGCGGGCGGTGTCGAGCCAACACACCGCCGCCCGCCGCC
GGGTGGAAGCCGCGCTCGCCGGCGATCTGGCGCTACGCGATCTGACTCCTGAGGAAGGCGTCGTGTTCAACGCCGAGATC
TCCGCCGCCATCGAAGAAAATCTGGCACACACCGATTACGGCCAGGTCCTCGCCGGGCGCGGCGTGACCACCGTCGCTCT
CGACGACGACGGTGCCATCGTGCGGTACCAACCGGACGGCACCACGACCGTGCTGGCTCCTGCGCAGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U9PRH2

Antitoxin

Source ID Structure
AlphaFold DB A0A2U9PRP8

References