Detailed information of TA system TA099162

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpA-brnA/BrnT_toxin-BrnA
• Location: 3231806..3232385
• Replicon: chromosome
• Accession: NZ_CP027527
• Organism: Magnetospirillum gryphiswaldense strain R3/S1

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A4U0V7
• Locus tag: MSR1L_RS15130
• Protein ID: WP_041634718.1
• Coordinates: 3232119..3232385 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: MSR1L_RS15125
• Protein ID: WP_024081481.1
• Coordinates: 3231806..3232132 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MSR1L_RS15100	3227507..3227863	+	357	WP_024081474.1	hypothetical protein	-
MSR1L_RS15105	3227886..3228359	+	474	WP_106003012.1	YchJ family protein	-
MSR1L_RS15110	3228368..3229312	+	945	WP_106003013.1	glycosyltransferase family 2 protein	-
MSR1L_RS15115	3229450..3229770	-	321	WP_106003839.1	hypothetical protein	-
MSR1L_RS15120	3229919..3231523	+	1605	WP_024081478.1	CHASE domain-containing protein	-
MSR1L_RS15125	3231806..3232132	-	327	WP_024081481.1	BrnA antitoxin family protein	Antitoxin
MSR1L_RS15130	3232119..3232385	-	267	WP_041634718.1	BrnT family toxin	Toxin
MSR1L_RS15140	3232805..3233230	+	426	WP_106003014.1	hemerythrin family protein	-
MSR1L_RS15145	3233276..3233539	-	264	WP_024081485.1	hypothetical protein	-
MSR1L_RS15150	3233596..3234045	-	450	WP_106003015.1	hypothetical protein	-
MSR1L_RS15155	3234163..3234381	+	219	WP_106003016.1	hypothetical protein	-
MSR1L_RS19935	3234553..3234924	-	372	WP_024081487.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-104)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10060.48 Da
• Isoelectric Point: 7.2028

>T99162 WP_041634718.1 NZ_CP027527:c3232385-3232119 [Magnetospirillum gryphiswaldense]
MPIEFDAVKNAINVAKHGVDFKVVDAFEWDTAVVVEDTRREYGETRLIALGYIGDRLHVLAFTIRDGNIRPISLRKANSR
EIRRYAEA

Nucleotide

• Length: 267 bp

>T99162 NZ_CP027527:c3232385-3232119 [Magnetospirillum gryphiswaldense]
ATGCCTATAGAATTCGACGCCGTTAAAAATGCCATCAACGTCGCCAAGCATGGCGTCGATTTTAAGGTGGTCGACGCATT
TGAATGGGACACAGCTGTGGTGGTCGAAGATACCCGCCGGGAATATGGCGAAACACGTCTAATCGCCCTTGGGTATATCG
GTGACCGCTTGCACGTACTGGCTTTTACCATCCGTGACGGGAACATTCGTCCCATCTCTTTGCGTAAAGCCAATTCCAGG
GAGATTAGGCGTTATGCCGAAGCTTAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 11692.27 Da
• Isoelectric Point: 6.3867

>AT99162 WP_024081481.1 NZ_CP027527:c3232132-3231806 [Magnetospirillum gryphiswaldense]
MPKLKPSTIIPTDEEDAAISAAISTDPDSPELTDANFANARPASEVLANYLGATETEALLRRRGRPLKADRKVDIKLRID
PDVVEALRSGGKGWQTRANEILRKGVGL

Nucleotide

• Length: 327 bp

>AT99162 NZ_CP027527:c3232132-3231806 [Magnetospirillum gryphiswaldense]
ATGCCGAAGCTTAAGCCATCGACCATCATACCGACCGACGAGGAAGATGCTGCAATTTCCGCTGCAATCAGCACCGATCC
AGATTCGCCAGAGTTGACCGATGCCAATTTTGCCAATGCCCGTCCTGCTTCCGAGGTTCTGGCAAATTATCTTGGTGCCA
CCGAGACTGAGGCGCTTTTGCGTCGTCGTGGTCGTCCGTTGAAAGCCGACCGCAAGGTGGACATCAAGCTGCGCATTGAT
CCTGATGTTGTTGAGGCTCTGCGGTCTGGTGGAAAGGGTTGGCAGACCCGTGCCAATGAGATTTTGCGTAAAGGCGTTGG
GCTGTAG

Structures

Toxin

• AlphaFold DB: A4U0V7