Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 14052..14577 Replicon plasmid unnamed
Accession NZ_CP027463
Organism Escherichia coli isolate 07-4299

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag C6W75_RS25170 Protein ID WP_001159871.1
Coordinates 14052..14357 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0F6YRL5
Locus tag C6W75_RS25175 Protein ID WP_038355956.1
Coordinates 14359..14577 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W75_RS25145 10222..10638 - 417 WP_001278818.1 plasmid partitioning/stability family protein -
C6W75_RS25150 10631..11611 - 981 WP_106912565.1 plasmid segregation protein ParM -
C6W75_RS25155 12025..12333 - 309 WP_000030199.1 hypothetical protein -
C6W75_RS25160 12420..13064 - 645 WP_001144036.1 ParA family protein -
C6W75_RS25165 13245..14051 - 807 WP_040116953.1 site-specific integrase -
C6W75_RS25170 14052..14357 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
C6W75_RS25175 14359..14577 - 219 WP_038355956.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C6W75_RS25180 15173..15433 + 261 WP_000343102.1 hypothetical protein -
C6W75_RS25185 15430..16020 + 591 WP_106912566.1 hypothetical protein -
C6W75_RS25190 16038..16385 - 348 WP_033817147.1 hypothetical protein -
C6W75_RS25200 16514..16849 + 336 WP_021292998.1 colicin transporter -
C6W75_RS25205 16856..17226 - 371 Protein_26 colicin -
C6W75_RS25210 17286..18017 - 732 WP_000850413.1 complement resistance protein TraT -
C6W75_RS26135 18294..18404 - 111 Protein_28 IS481 family transposase -
C6W75_RS26140 18408..18622 - 215 Protein_29 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - hlyD / hlyB / hlyA / hlyC 1..125059 125059


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T99094 WP_001159871.1 NZ_CP027463:c14357-14052 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T99094 NZ_CP027463:c14357-14052 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTATCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8400.36 Da        Isoelectric Point: 4.7405

>AT99094 WP_038355956.1 NZ_CP027463:c14577-14359 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWRAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT99094 NZ_CP027463:c14577-14359 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGTGCTGAACGCTGGCGGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6YRL5

References