Detailed information of TA system TA099093

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4504765..4505367
• Replicon: chromosome
• Accession: NZ_CP027462
• Organism: Escherichia coli isolate 07-4299

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: C6W75_RS23340
• Protein ID: WP_000897305.1
• Coordinates: 4505056..4505367 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6W75_RS23335
• Protein ID: WP_000356397.1
• Coordinates: 4504765..4505055 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W75_RS23305	4500691..4501593	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
C6W75_RS23310	4501590..4502225	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
C6W75_RS23315	4502222..4503151	+	930	WP_000027706.1	formate dehydrogenase accessory protein FdhE	-
C6W75_RS23320	4503481..4503723	-	243	WP_001087409.1	hypothetical protein	-
C6W75_RS23325	4503942..4504160	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
C6W75_RS23335	4504765..4505055	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
C6W75_RS23340	4505056..4505367	-	312	WP_000897305.1	hypothetical protein	Toxin
C6W75_RS23345	4505596..4506504	+	909	WP_001162704.1	alpha/beta hydrolase	-
C6W75_RS23350	4506568..4507509	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
C6W75_RS23355	4507554..4507991	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
C6W75_RS23360	4507988..4508860	-	873	WP_000920762.1	virulence factor BrkB family protein	-
C6W75_RS23365	4508854..4509453	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T99093 WP_000897305.1 NZ_CP027462:c4505367-4505056 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T99093 NZ_CP027462:c4505367-4505056 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT99093 WP_000356397.1 NZ_CP027462:c4505055-4504765 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT99093 NZ_CP027462:c4505055-4504765 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7