Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3630814..3631355 Replicon chromosome
Accession NZ_CP027462
Organism Escherichia coli isolate 07-4299

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag C6W75_RS18970 Protein ID WP_000615976.1
Coordinates 3631077..3631355 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag C6W75_RS18965 Protein ID WP_000729704.1
Coordinates 3630814..3631074 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W75_RS18940 3626597..3627382 - 786 WP_000207574.1 putative lateral flagellar export/assembly protein LafU -
C6W75_RS18945 3627354..3629066 + 1713 Protein_3475 flagellar biosynthesis protein FlhA -
C6W75_RS18950 3629171..3629449 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
C6W75_RS18955 3629442..3629798 + 357 WP_001030482.1 type II toxin-antitoxin system HicB family antitoxin -
C6W75_RS18960 3629855..3630628 - 774 WP_000543899.1 C40 family peptidase -
C6W75_RS18965 3630814..3631074 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
C6W75_RS18970 3631077..3631355 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
C6W75_RS18975 3631511..3632251 + 741 WP_001225679.1 murein L,D-transpeptidase -
C6W75_RS18980 3632222..3632989 - 768 WP_000333380.1 class II glutamine amidotransferase -
C6W75_RS18985 3633195..3633773 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T99090 WP_000615976.1 NZ_CP027462:3631077-3631355 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T99090 NZ_CP027462:3631077-3631355 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT99090 WP_000729704.1 NZ_CP027462:3630814-3631074 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT99090 NZ_CP027462:3630814-3631074 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References