Detailed information of TA system TA099086

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2140254..2140780
• Replicon: chromosome
• Accession: NZ_CP027462
• Organism: Escherichia coli isolate 07-4299

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: C6W75_RS11135
• Protein ID: WP_000323025.1
• Coordinates: 2140493..2140780 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: C6W75_RS11130
• Protein ID: WP_000534858.1
• Coordinates: 2140254..2140493 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W75_RS11095	2135588..2135944	+	357	WP_001374839.1	class I SAM-dependent methyltransferase	-
C6W75_RS26050	2136555..2136842	+	288	Protein_2037	hypothetical protein	-
C6W75_RS11110	2137611..2138952	+	1342	Protein_2038	ISNCY family transposase	-
C6W75_RS11120	2139389..2139721	-	333	WP_001317460.1	FlxA-like family protein	-
C6W75_RS11125	2139924..2140229	-	306	WP_001326990.1	hypothetical protein	-
C6W75_RS11130	2140254..2140493	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
C6W75_RS11135	2140493..2140780	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
C6W75_RS11140	2140852..2141007	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
C6W75_RS11145	2141224..2141475	+	252	WP_000980994.1	hypothetical protein	-
C6W75_RS11150	2141542..2141820	+	279	WP_012304870.1	hypothetical protein	-
C6W75_RS11155	2141822..2142871	+	1050	WP_001265198.1	DUF968 domain-containing protein	-
C6W75_RS11160	2142885..2143637	+	753	WP_001047135.1	antitermination protein	-
C6W75_RS25835	2143915..2144004	-	90	WP_120795389.1	hypothetical protein	-
C6W75_RS11165	2144059..2144271	-	213	WP_000087756.1	cold shock-like protein CspF	-
C6W75_RS11170	2144572..2144787	+	216	WP_000066484.1	cold shock-like protein CspB	-
C6W75_RS26210	2145151..2145321	-	171	WP_211180497.1	putative zinc-binding protein YnfU	-
C6W75_RS11175	2145541..2145756	+	216	WP_000839590.1	phage lysis protein EssD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2126982..2176164	49182

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T99086 WP_000323025.1 NZ_CP027462:2140493-2140780 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T99086 NZ_CP027462:2140493-2140780 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT99086 WP_000534858.1 NZ_CP027462:2140254-2140493 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT99086 NZ_CP027462:2140254-2140493 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8