Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1664134..1664636 Replicon chromosome
Accession NZ_CP027462
Organism Escherichia coli isolate 07-4299

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C6W75_RS08485 Protein ID WP_000767829.1
Coordinates 1664382..1664636 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C6W75_RS08480 Protein ID WP_001259255.1
Coordinates 1664134..1664385 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W75_RS08450 1659312..1660379 - 1068 WP_000080096.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
C6W75_RS08455 1660379..1661449 - 1071 WP_001297360.1 histidinol-phosphate transaminase -
C6W75_RS08460 1661446..1662750 - 1305 WP_047083378.1 histidinol dehydrogenase -
C6W75_RS08465 1662756..1663655 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
C6W75_RS08475 1663801..1663851 - 51 WP_001364200.1 his operon leader peptide -
C6W75_RS08480 1664134..1664385 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C6W75_RS08485 1664382..1664636 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C6W75_RS08490 1664719..1665543 + 825 WP_000754749.1 SDR family oxidoreductase -
C6W75_RS08495 1665589..1666518 + 930 WP_000803350.1 LysR family transcriptional regulator -
C6W75_RS08500 1666733..1666795 + 63 WP_010723108.1 membrane protein YoeI -
C6W75_RS08505 1666785..1668143 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C6W75_RS08510 1668322..1669380 + 1059 WP_000492304.1 transport protein YeeE -
C6W75_RS08515 1669394..1669621 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T99077 WP_000767829.1 NZ_CP027462:1664382-1664636 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T99077 NZ_CP027462:1664382-1664636 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT99077 WP_001259255.1 NZ_CP027462:1664134-1664385 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT99077 NZ_CP027462:1664134-1664385 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References