Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 136096..136727 Replicon chromosome
Accession NZ_CP027462
Organism Escherichia coli isolate 07-4299

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag C6W75_RS00640 Protein ID WP_001259388.1
Coordinates 136096..136371 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag C6W75_RS00645 Protein ID WP_000593555.1
Coordinates 136368..136727 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W75_RS00625 131100..132167 + 1068 WP_000361467.1 HlyD family secretion protein -
C6W75_RS00630 132164..134899 + 2736 WP_000149124.1 ribosome-associated ATPase/putative transporter RbbA -
C6W75_RS00635 134899..136023 + 1125 WP_001216257.1 ABC transporter permease -
C6W75_RS00640 136096..136371 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
C6W75_RS00645 136368..136727 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C6W75_RS00650 136847..137248 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
C6W75_RS00655 137254..138060 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
C6W75_RS00660 138057..138821 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
C6W75_RS00665 138821..139654 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
C6W75_RS00670 139651..140595 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 129112..137248 8136


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T99072 WP_001259388.1 NZ_CP027462:136096-136371 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T99072 NZ_CP027462:136096-136371 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT99072 WP_000593555.1 NZ_CP027462:136368-136727 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT99072 NZ_CP027462:136368-136727 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References