Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 5055156..5055810 Replicon chromosome
Accession NZ_CP027459
Organism Escherichia coli strain 90-3040

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag C6W76_RS27245 Protein ID WP_000244777.1
Coordinates 5055403..5055810 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag C6W76_RS27240 Protein ID WP_000354046.1
Coordinates 5055156..5055422 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W76_RS27215 5050326..5051069 + 744 WP_000951948.1 SDR family oxidoreductase -
C6W76_RS27220 5051126..5052559 - 1434 WP_032207745.1 6-phospho-beta-glucosidase BglA -
C6W76_RS27225 5052604..5052914 + 311 Protein_4968 N(4)-acetylcytidine aminohydrolase -
C6W76_RS27230 5053078..5053737 + 660 WP_000250274.1 hemolysin III family protein -
C6W76_RS27235 5053933..5054913 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
C6W76_RS27240 5055156..5055422 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
C6W76_RS27245 5055403..5055810 + 408 WP_000244777.1 toxin CptA Toxin
C6W76_RS27250 5055850..5056371 - 522 WP_001055874.1 flavodoxin FldB -
C6W76_RS27255 5056483..5057379 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
C6W76_RS27260 5057403..5058113 + 711 WP_000715227.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C6W76_RS27265 5058119..5059851 + 1733 Protein_4976 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T99043 WP_000244777.1 NZ_CP027459:5055403-5055810 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T99043 NZ_CP027459:5055403-5055810 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT99043 WP_000354046.1 NZ_CP027459:5055156-5055422 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT99043 NZ_CP027459:5055156-5055422 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References