Detailed information of TA system TA099039

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 3423721..3424424
• Replicon: chromosome
• Accession: NZ_CP027459
• Organism: Escherichia coli strain 90-3040

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A8T6BCS1
• Locus tag: C6W76_RS18690
• Protein ID: WP_001616568.1
• Coordinates: 3423721..3424062 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: C6W76_RS18695
• Protein ID: WP_032208435.1
• Coordinates: 3424083..3424424 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W76_RS18660	3419136..3420116	+	981	WP_032208436.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
C6W76_RS18665	3420124..3420246	-	123	WP_001300013.1	hypothetical protein	-
C6W76_RS18675	3420638..3421824	-	1187	Protein_3423	IS3-like element IS2 family transposase	-
C6W76_RS18685	3422466..3423473	-	1008	WP_001616572.1	restriction endonuclease	-
C6W76_RS18690	3423721..3424062	-	342	WP_001616568.1	TA system toxin CbtA family protein	Toxin
C6W76_RS18695	3424083..3424424	-	342	WP_032208435.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C6W76_RS18700	3424435..3424977	-	543	WP_001616564.1	DNA repair protein RadC	-
C6W76_RS18705	3424990..3425430	-	441	WP_001616563.1	antirestriction protein	-
C6W76_RS18710	3425461..3426282	-	822	WP_032208434.1	DUF945 domain-containing protein	-
C6W76_RS18715	3426380..3426610	-	231	WP_072057388.1	DUF905 domain-containing protein	-
C6W76_RS18720	3426680..3427129	-	450	WP_032208432.1	hypothetical protein	-
C6W76_RS18725	3427126..3427581	-	456	WP_032208430.1	hypothetical protein	-
C6W76_RS18730	3427620..3428255	-	636	WP_001616559.1	hypothetical protein	-
C6W76_RS18735	3428252..3428965	-	714	WP_106913948.1	transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (13-112)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12746.75 Da
• Isoelectric Point: 9.6548

>T99039 WP_001616568.1 NZ_CP027459:c3424062-3423721 [Escherichia coli]
MKTLPATTPQAAKLCLSPVAVWQMLLARLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYGLVRIDRRGF
NWQEQSPYLRAVDILRARQATGLLKRNRISAAR

Nucleotide

• Length: 342 bp

>T99039 NZ_CP027459:c3424062-3423721 [Escherichia coli]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGCGAAGCTATGTCTGTCGCCCGTGGCAGTGTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACGCCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAGGGGATTC
AACTGGCAAGAACAGTCCCCATATCTGCGGGCGGTCGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTAAAAAGAAA
TCGTATTAGCGCGGCACGGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12827.46 Da
• Isoelectric Point: 6.4660

>AT99039 WP_032208435.1 NZ_CP027459:c3424424-3424083 [Escherichia coli]
MKSTDSENDSFLKWGLNRNVTPCFGARLVQEGNRLHYLADRASITGKFSEAECLRLDVAFPHFISQMESMLTTGEMNPRH
AHCVTLYYNGFTCEADTLGSYGYVYIAVYPTQR

Nucleotide

• Length: 342 bp

>AT99039 NZ_CP027459:c3424424-3424083 [Escherichia coli]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGGCTGCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTCAGCGAAGCAGAATGTC
TTAGGCTGGATGTGGCATTCCCACATTTTATCAGCCAGATGGAGTCGATGTTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTGTGTCACCCTGTACTACAATGGTTTCACCTGCGAAGCCGATACCCTTGGTAGTTACGGCTACGTATACATCGC
CGTTTACCCCACTCAACGTTAA