Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 32984..33509 Replicon plasmid unnamed
Accession NZ_CP027458
Organism Escherichia coli strain 88-3493

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag C6W79_RS25805 Protein ID WP_001159871.1
Coordinates 32984..33289 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag C6W79_RS25810 Protein ID WP_000813639.1
Coordinates 33291..33509 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W79_RS25770 28251..29521 + 1271 Protein_45 translesion error-prone DNA polymerase V subunit UmuC -
C6W79_RS25780 29526..29918 - 393 WP_000340836.1 plasmid partitioning/stability family protein -
C6W79_RS25785 29923..30894 - 972 WP_001103695.1 hypothetical protein -
C6W79_RS25790 31123..31767 + 645 WP_000633912.1 AAA family ATPase -
C6W79_RS25795 31761..32036 + 276 WP_000239527.1 hypothetical protein -
C6W79_RS25800 32174..32983 - 810 WP_000016958.1 site-specific integrase -
C6W79_RS25805 32984..33289 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
C6W79_RS25810 33291..33509 - 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C6W79_RS25815 34105..34365 + 261 WP_000343084.1 hypothetical protein -
C6W79_RS25820 34362..34952 + 591 WP_106888579.1 hypothetical protein -
C6W79_RS25825 34970..35317 - 348 WP_000142428.1 hypothetical protein -
C6W79_RS25835 35502..35780 + 279 WP_001365566.1 hypothetical protein -
C6W79_RS25840 35787..36122 - 336 Protein_57 colicin -
C6W79_RS25845 36217..36948 - 732 WP_000850413.1 complement resistance protein TraT -
C6W79_RS26625 37225..37335 - 111 Protein_59 IS481 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - hlyD / hlyB / hlyA / hlyC 1..107796 107796


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T99021 WP_001159871.1 NZ_CP027458:c33289-32984 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T99021 NZ_CP027458:c33289-32984 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCTGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT99021 WP_000813639.1 NZ_CP027458:c33509-33291 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT99021 NZ_CP027458:c33509-33291 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References